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Trace element solution

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Internal Solution-ID: S3924

This solution is used in 6 media and was first defined in medium J243.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 12.800 g 12.8 66.967
FeCl3 x 6 H2O 1.350 g 1.35 4.995
MnCl2 x 4 H2O 0.100 g 0.1 -
CoCl2 x 6 H2O 0.024 g 0.024 0.185
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnCl2 0.100 g 0.1 0.734
CuCl2 x 2 H2O 0.025 g 0.025 0.147
H3BO3 0.010 g 0.01 0.162
NaCl 1.000 g 1 17.112
NiCl2 x 6 H2O 0.120 g 0.12 0.926
Na2SeO3 x 5 H2O 0.026 g 0.026 0.099
Distilled water 1000.000 ml - -
1 Dissolve nitrilotriacetic acid first in 200 ml of distilled water, and adjust pH to 6.5 with KOH. Then add the remaining salts and adjust volume to 1.0 L. Readjust pH to 7.0 with KOH.
2 Comment: For strain JCM 10134, incubate with a 10-20% inoculum.
J243 METHANOTHRIX MEDIUM
J272 JCM MEDIUM No. 272
J335 JCM MEDIUM No. 335
J807 WR MEDIUM FOR METHANOBACTERIACEAE
J881 KNH MEDIUM
J907 GEOBACTER MEDIUM