MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

JCM 807 . WR MEDIUM FOR METHANOBACTERIACEAE

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J807 #

ml
Equipment needed: Hungate tubes Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.136 g 0.133 0.979
NH4Cl 0.535 g 0.524 9.796
MgCl2 x 6 H2O 0.204 g 0.2 0.983
CaCl2 x 2 H2O 0.147 g 0.144 0.979
NaHCO3 2.520 g 2.468 29.381
Tryptone
(BD-Difco) 		1.000 g 0.979 13.779
Sodium acetate 0.820 g 0.803 9.791
Trace element solution 10.000 ml - -
Trace vitamins 10.000 ml - -
Se/W solution 1.000 ml - -
L-Cysteine HCl x H2O 0.120 g 0.118 -
Na2S x 9 H2O 0.120 g 0.118 0.489
Resazurin 1.000 mg 9.8e-4 0.004
Distilled water 1000.000 ml - -
1 Mix ingredients except NaHCO3, L-cysteine x HCl x H2O and Na2S x 9H2O, and adjust pH to 7.0. Bring to a boil for 5-10 sec, then, cool down under a stream of H2-CO2 (80:20, v/v) and add NaHCO3. Dispense the medium into suitable culture vessels (e.g., 5 ml in Hungate tubes) under the same gas mixture and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Separately autoclave L-cysteine x HCl x H2O and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add L-cysteine x HCl x H2O and Na2S x 9H2O solutions. Readjust pH to 6.7, if necessary. Pressurize the inoculated bottles to 150 kPa H2-CO2 (80:20, v/v).

Trace element solution #

1000
Solution occurs in 5 other media (first defined in Medium J243).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 12.800 g 12.8 66.967
FeCl3 x 6 H2O 1.350 g 1.35 4.995
MnCl2 x 4 H2O 0.100 g 0.1 -
CoCl2 x 6 H2O 0.024 g 0.024 0.185
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnCl2 0.100 g 0.1 0.734
CuCl2 x 2 H2O 0.025 g 0.025 0.147
H3BO3 0.010 g 0.01 0.162
NaCl 1.000 g 1 17.112
NiCl2 x 6 H2O 0.120 g 0.12 0.926
Na2SeO3 x 5 H2O 0.026 g 0.026 0.099
Distilled water 1000.000 ml - -
1 Dissolve nitrilotriacetic acid first in 200 ml of distilled water, and adjust pH to 6.5 with KOH. Then add the remaining salts and adjust volume to 1.0 L. Readjust pH to 7.0 with KOH.
2 Comment: For strain JCM 10134, incubate with a 10-20% inoculum.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Se/W solution #

1000
Solution occurs in 8 other media (first defined in Medium J284).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SeO3 x 5 H2O 4 mg 0.004 0.015
Na2WO4 x 2 H2O 4 mg 0.004 0.012
Distilled water 1000 ml - -

Metadata

Last modified: 03.04.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Hungate tubes
Edit in Medium builder

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 2.468 29.381
Tryptone 0.979 13.779
Sodium acetate 0.803 9.791
NH4Cl 0.524 9.796
MgCl2 x 6 H2O 0.2 0.983
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
Show more