Mineral medium
Internal Solution-ID: S4314
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Sea Salt
(Sigma) |
25.00 | g | 27.747 | - | |
| NH4Cl | 0.25 | g | 0.277 | 5.187 | |
| Na2SO4 | 3.50 | g | 3.885 | 27.348 | |
| Resazurin | 1.00 | mg | 0.001 | 0.005 | |
| Distilled water | 900.00 | ml | - | - | |
| 1 Mix components and adjust pH to 7.2 - 7.4. Boil Mineral medium and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute Mineral medium into culture vessels (e.g., 9.0 ml of the medium in tubes) under the same gas mixture, seal with Teflon-coated rubber stoppers and autoclave. After cooling, add the following components (per 9.0 ml Mineral medium) from sterile anoxic stock solutions (autoclaved or *filter-sterilized): | |||||
|
NaHCO3
(*, 8.5%) |
0.40 | ml | - | - | |
|
Sodium pyruvate
(*, 2%) |
0.50 | ml | - | - | |
|
Trace vitamins (see Medium No. 197)
(*) |
0.10 | ml | - | - | |
| Decane | 0.01 | ml | - | - | |
|
Yeast extract
(1%) |
0.05 | ml | - | - | |
| Reducing agent solution | 0.08 | ml | - | - | |
Media with this solution
| J521 | MODIFIED DESULFOGLAEBA ALKANEXEDENS MEDIUM |