Name: MODIFIED DESULFOGLAEBA ALKANEXEDENS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Main sol. J521 #

Mineral medium #

901

Equipment needed: Filter Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Sea Salt (Sigma)	25.00	g	27.747	-
NH₄Cl	0.25	g	0.277	5.187
Na₂SO₄	3.50	g	3.885	27.348
Resazurin	1.00	mg	0.001	0.005
Distilled water	900.00	ml	-	-
1 Mix components and adjust pH to 7.2 - 7.4. Boil Mineral medium and cool down under a N₂-CO₂ (4:1, v/v) gas stream. Distribute Mineral medium into culture vessels (e.g., 9.0 ml of the medium in tubes) under the same gas mixture, seal with Teflon-coated rubber stoppers and autoclave. After cooling, add the following components (per 9.0 ml Mineral medium) from sterile anoxic stock solutions (autoclaved or *filter-sterilized):
NaHCO₃ (*, 8.5%)	0.40	ml	-	-
Sodium pyruvate (*, 2%)	0.50	ml	-	-
Trace vitamins (see Medium No. 197) (*)	0.10	ml	-	-
Decane	0.01	ml	-	-
Yeast extract (1%)	0.05	ml	-	-
Reducing agent solution	0.08	ml	-	-

1000

Solution occurs in 1 other media (first defined in Medium J521).

Equipment needed: Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Na₂S x 9 H₂O	12.5	g	12.5	52.044
L-Cysteine HCl x H₂O	12.5	g	12.5	-
Distilled water	1000.0	ml	-	-
1 Autoclave and store under a N₂ atmosphere.
2 Comment: For strain JCM 13588, inoculate with a 20% inoculum, and incubate the vessels in inverted position.