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Reducing agent solution

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Internal Solution-ID: S4315

This solution is used in 2 media and was first defined in medium J521.

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 12.5 g 12.5 52.044
L-Cysteine HCl x H2O 12.5 g 12.5 -
Distilled water 1000.0 ml - -
1 Autoclave and store under a N2 atmosphere.
2 Comment: For strain JCM 13588, inoculate with a 20% inoculum, and incubate the vessels in inverted position.
J521 MODIFIED DESULFOGLAEBA ALKANEXEDENS MEDIUM
J1097 SEAWATER PYRUVATE THIOSULFATE MEDIUM