Main sol. J881
Internal Solution-ID: S4868
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.136 | g | 0.136 | 0.996 | |
| NH4Cl | 0.535 | g | 0.533 | 9.972 | |
| MgCl2 x 6 H2O | 3.050 | g | 3.041 | 14.957 | |
| CaCl2 x 2 H2O | 0.147 | g | 0.147 | 0.997 | |
| NaHCO3 | 2.520 | g | 2.512 | 29.908 | |
| NaCl | 20.450 | g | 20.389 | 348.885 | |
| Na2SO4 | 2.840 | g | 2.832 | 19.935 | |
| Sodium acetate | 0.656 | g | 0.654 | 7.973 | |
| Trace element solution | 1.000 | ml | - | - | |
| Se/W solution | 1.000 | ml | - | - | |
| Trace vitamins | 1.000 | ml | - | - | |
| Resazurin | 1.000 | mg | 1.0e-3 | 0.004 | |
| Sodium thioglycolate | 0.100 | g | 0.1 | 0.874 | |
| Ascorbic acid | 0.100 | g | 0.1 | 0.566 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Mix all ingredients except sodium thioglycolate and ascorbic acid, and expel dissolving O2 from the solution with deoxygenated N2-CO2 (80:20, v/v). Dispense the solution into vials where deoxygenated H2-CO2 (80:20, v/v) is sparged. Cap with butyl rubber stoppers. Autoclave, then add reducing agent solution (sodium thioglycolate and ascorbic acid, 1% solution each) sterilized with 0.2 �m-filter (0.1 ml in 10 ml medium) and inoculate fully grown culture at 5-10% of final volume. Fill with H2-CO2 (80:20, v/v) gas at 1.5 atm. | |||||
Media with this solution
| J881 | KNH MEDIUM |