Modified Hutner's basal salts
Internal Solution-ID: S4899
This solution is used in 4 media and was first defined in medium J900.
Internal Solution-ID: S4899
This solution is used in 4 media and was first defined in medium J900.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid
(NTA) |
10.000 | g | 10 | 52.318 | |
MgSO4 x 7 H2O | 29.700 | g | 29.7 | 120.499 | |
CaCl2 x 2 H2O | 3.335 | g | 3.335 | 22.685 | |
(NH4)6Mo7O24 x 4 H2O | 9.250 | mg | 0.009 | - | |
FeSO4 x 7 H2O | 99.000 | mg | 0.099 | 0.356 | |
Metals ''44'' | 50.000 | ml | - | - | |
Distilled water | 950.000 | ml | - | - | |
1 Dissolve nitrilotriacetic acid and adjust pH to 7.0 with KOH (about 7.3 g). Then proceed to add salts. Readjust pH to 6.8 with NaOH or H2SO4. |