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RST trace elements

Internal Solution-ID: S4945

This solution is used in 2 media and was first defined in medium J933.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 2.00 g 2 10.464
MnSO4 x n H2O 1.00 g 1 -
Fe(NH4)2(SO4)2 x 6 H2O 0.80 g 0.8 -
CoCl2 x 6 H2O 0.20 g 0.2 1.54
ZnSO4 x 7 H2O 0.20 g 0.2 0.696
CuCl2 x 2 H2O 0.02 g 0.02 0.117
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.02 g 0.02 0.083
Na2SeO4 0.02 g 0.02 0.106
Na2WO4 x 2 H2O 0.02 g 0.02 0.061
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.0 with KOH. Then proceed to add minerals.
J933 THERMOANAEROBACULUM AQUATICUM MEDIUM
J1097 SEAWATER PYRUVATE THIOSULFATE MEDIUM