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Main sol. J1064

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Internal Solution-ID: S5129

Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KCl 0.50 g 0.499 6.694
KH2PO4 0.20 g 0.2 1.467
NaCl 1.00 g 0.998 17.077
NH4Cl 0.05 g 0.05 0.933
CaCl2 x 2 H2O 0.10 g 0.1 0.679
MgCl2 x 6 H2O 0.40 g 0.399 1.964
Yeast extract 1.00 g 0.998 -
Glucose 0.36 g 0.359 1.994
Trace vitamins 1.00 ml - -
Trace element solution 1.00 ml - -
NaHCO3 0.84 g 0.838 9.979
Na2S x 9 H2O 0.50 g 0.499 2.078
Resazurin 1.00 mg 1.0e-3 0.004
Distilled water 1000.00 ml - -
1 Mix ingredients except NaHCO3, Trace vitamins and Na2S x 9H2O, and autoclave under a N2-CO2 (90:10) atmosphere. Filter-sterilize NaHCO3 as an 8.4% solution and Trace vitamins while gassing with N2. Separately autoclave Na2S x 9H2O as 5% solution under a N2 atmosphere. Aseptically and anaerobically add NaHCO3 and Trace vitamins to the medium under a N2-CO2 (90:10) atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O solution, and adjust pH to 7.5 - 8.0.
J1064 GEOSPOROBACTER MEDIUM