Main sol. J1101
Internal Solution-ID: S5187
Equipment needed: Hungate tubes
Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 10.0 | g | 9.891 | 169.254 | |
| KCl | 0.2 | g | 0.198 | 2.654 | |
| K2HPO4 | 0.2 | g | 0.198 | 1.136 | |
| MgCl2 x 6 H2O | 0.1 | g | 0.099 | 0.487 | |
| NH4Cl | 1.0 | g | 0.989 | 18.492 | |
| Yeast extract | 0.1 | g | 0.099 | - | |
| Trace vitamins | 2.0 | ml | - | - | |
| Trace minerals solution | 1.0 | ml | - | - | |
| Resazurin | 0.5 | mg | 4.9e-4 | 0.002 | |
| Distilled water | 680.0 | ml | - | - | |
| 1 Mix components thoroughly and autoclave under a N2 atmosphere in a bottle capped with a butyl rubber stopper. After cooling, add the following solution to the medium: | |||||
| Alkaline solution | 300.0 | ml | - | - | |
|
Glucose
(autoclaved, 1.0 M) |
20.0 | ml | - | - | |
| 2 Anaerobically and aseptically distribute the medium into culture vessels (e.g., 10 ml medium in Hungate tubes) under a N2 gas atmosphere and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution (autoclaved and stored under a N2 gas atmosphere): | |||||
|
Na2S x 9 H2O
(5%) |
8.0 | ml | - | - | |
| 3 Adjust pH to 9.0, if necessary. | |||||
Media with this solution
| J1101 | HALOLACTIBACILLUS 9G MEDIUM |