Main sol. J1292
Internal Solution-ID: S5442
Equipment needed: Anaerobic workbench
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 0.3 | g | 0.294 | 5.028 | |
| KCl | 0.1 | g | 0.098 | 1.314 | |
| NH4Cl | 0.3 | g | 0.294 | 5.493 | |
| KH2PO4 | 0.3 | g | 0.294 | 2.159 | |
| Na2HPO4 | 0.3 | g | 0.294 | 2.07 | |
| MgCl2 x 6 H2O | 0.2 | g | 0.196 | 0.964 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.098 | 0.666 | |
| Yeast extract | 5.0 | g | 4.897 | - | |
| Beef extract | 3.0 | g | 2.938 | - | |
| Trace element solution | 1.0 | ml | - | - | |
| Resazurin | 0.5 | mg | 4.9e-4 | 0.002 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.49 | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Mix components thoroughly and adjust pH to 7.0. Distribute the medium into culture vessels (e.g., 20 ml medium in 60 ml serum bottle) under a N2 gas atmosphere, seal with butyl rubber stoppers and autoclave.After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved and stored under N2) | |||||
|
Sodium pyruvate
(1.0 M) |
10.0 | ml | - | - | |
|
Rumen fluid
, clarified
(see Medium No. 266) |
10.0 | ml | - | - | |
Media with this solution
| J1292 | MQ116 MEDIUM |