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Trace element solution

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Internal Solution-ID: S3980

This solution is used in 8 media and was first defined in medium J284.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 12.800 g 12.8 66.967
FeCl3 x 6 H2O 1.350 g 1.35 4.995
MnCl2 x 4 H2O 0.100 g 0.1 -
CoCl2 x 6 H2O 0.024 g 0.024 0.185
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnCl2 0.100 g 0.1 0.734
CuCl2 x 2 H2O 0.025 g 0.025 0.147
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.024 g 0.024 0.099
NiCl2 x 6 H2O 0.120 g 0.12 0.926
Distilled water 1000.000 ml - -
1 Dissolve nitrilotriacetic acid first in 200 ml of distilled water and adjust pH to 6.5 with KOH. Then add the remaining salts and bring volume to 1.0 L. Readjust pH to 7.0 with KOH.
J284 SI MEDIUM
J317 JCM MEDIUM No. 317
J846 MO-CFXI MEDIUM
J871 PROTEINIPHILUS 5MH MEDIUM
J920 HYDROGENISPORA MEDIUM
J936 LX-D MEDIUM
J1219 METHYLOTROPH MEDIUM
J1292 MQ116 MEDIUM