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JCM 1219 . METHYLOTROPH MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1219 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.270 g 0.269 1.974
NaNO3 0.420 g 0.418 4.917
MgCl2 x 6 H2O 0.200 g 0.199 0.979
CaCl2 x 2 H2O 0.070 g 0.07 0.474
Na2SO4 0.014 g 0.014 0.098
HEPES 4.770 g 4.746 19.916
Trace element solution 10.000 ml - -
Se/W solution 10.000 ml - -
Trace vitamins 10.000 ml - -
Distilled water 970.000 ml - -
1 Mix components and adjust pH to 7.0 with 6 N KOH. For preparation of solid medium, add 15.0 g agar. Autoclave. After cooling, add the following solutions (filer-sterilized):
Methanol 0.810 ml - -
CuCl2
(10 mM) 		2.000 ml - -
CeCl3
(10 mM) 		2.000 ml - -

Trace element solution #

1000
Solution occurs in 7 other media (first defined in Medium J284).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 12.800 g 12.8 66.967
FeCl3 x 6 H2O 1.350 g 1.35 4.995
MnCl2 x 4 H2O 0.100 g 0.1 -
CoCl2 x 6 H2O 0.024 g 0.024 0.185
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnCl2 0.100 g 0.1 0.734
CuCl2 x 2 H2O 0.025 g 0.025 0.147
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.024 g 0.024 0.099
NiCl2 x 6 H2O 0.120 g 0.12 0.926
Distilled water 1000.000 ml - -
1 Dissolve nitrilotriacetic acid first in 200 ml of distilled water and adjust pH to 6.5 with KOH. Then add the remaining salts and bring volume to 1.0 L. Readjust pH to 7.0 with KOH.

Se/W solution #

1000
Solution occurs in 8 other media (first defined in Medium J284).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SeO3 x 5 H2O 4 mg 0.004 0.015
Na2WO4 x 2 H2O 4 mg 0.004 0.012
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
HEPES 4.746 19.916
NaNO3 0.418 4.917
KH2PO4 0.269 1.974
MgCl2 x 6 H2O 0.199 0.979
Nitrilotriacetic acid 0.127 0.666
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
temperature mesophilic 3 strains