Main sol. 318b
Internal Solution-ID: S591
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.30 | g | 0.296 | 2.176 | |
NaCl | 0.60 | g | 0.592 | 10.135 | |
MgCl2 x 6 H2O | 0.10 | g | 0.099 | 0.486 | |
CaCl2 x 2 H2O | 0.08 | g | 0.079 | 0.537 | |
Trace element solution | 10.00 | ml | - | - | |
NH4Cl | 1.00 | g | 0.987 | 18.455 | |
Yeast extract
(OXOID) |
0.50 | g | 0.494 | - | |
Trypticase peptone
(BD BBL) |
0.50 | g | 0.494 | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
KHCO3 | 4.00 | g | 3.949 | 39.441 | |
Ethanol | 1.30 | ml | - | - | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
L-Cysteine HCl x H2O | 0.30 | g | 0.296 | 1.686 | |
Na2S x 9 H2O | 0.30 | g | 0.296 | 1.233 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients (except bicarbonate, ethanol, vitamins, cysteine and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate and adjust pH to 7.0, then dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add ethanol, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins are prepared under 100% N2 gas and sterilized by filtration. Adjust pH of complete medium to 7.0 - 7.2, if necessary. |
318b | ANAEROTIGNUM NEOPROPIONICUM MEDIUM |