Main sol. 1840
Internal Solution-ID: S6431
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 240.00 | g | 240 | 4106.78 | |
| K2HPO4 | 2.50 | g | 2.5 | 14.353 | |
| KCl | 5.00 | g | 5 | 67.069 | |
| NH4Cl | 0.20 | g | 0.2 | 3.739 | |
| Trace element solution | 1.00 | ml | - | - | |
| Distilled water | 999.00 | ml | - | - | |
| 1 Adjust pH to 6.8 with 0.5 M KH2PO4. | |||||
| 2 Dispense into serum bottles (30 ml medium in 100 ml serum bottles, OR 15 ml in 50 serum bottles). | |||||
| 3 add 20% CO2 to gas phase (in serum bottles) | |||||
| 4 Autoclave at 121 °C for 20 min. | |||||
| 5 Before inoculation add from sterile stock solutions per liter: | |||||
| Vitamine mixture | 0.50 | ml/l | - | - | |
|
MgSO4 x 7 H2O
(1M in water) |
0.08 | ml/l | 0.02 | 0.08 | |
| alkaline trace metal solution | 0.50 | ml/l | - | - | |
|
Na-thiosulfate
(2M in water) |
10.00 | ml/l | 3.162 | 20 | |
|
NaHCO3
(8% in water) |
10.50 | ml/L | 0.84 | 9.999 | |
Media with this solution
| 1840 | Halorutilales haloarchaeon medium |