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Main sol. 477

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Internal Solution-ID: S975

Equipment needed: Autoclave Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KCl 0.33 g 0.329 4.418
MgCl2 x 6 H2O 2.75 g 2.745 13.5
MgSO4 x 7 H2O 3.45 g 3.443 13.969
NH4Cl 1.25 g 1.248 23.322
CaCl2 x 2 H2O 0.14 g 0.14 0.95
K2HPO4 0.14 g 0.14 0.802
KH2PO4 0.14 g 0.14 1.027
NaCl 0.50 g 0.499 8.539
Modified Wolin's mineral solution 10.00 ml - -
NiCl2 x 6 H2O
(0.1% w/v) 		2.00 ml 0.002 0.015
FeSO4 x 7 H2O
(40% w/v) 		50.00 ml 19.96 71.795
Distilled water 940.00 ml - -
1 Dissolve ingredients except ferrous sulfate and adjust pH to 2.5 with 4 N H2SO4. Sterilize medium by autoclaving for 15 min at 121°C. Prior to inoculation add FeSO4 x 7 H2O from a 40% (w/v) stock solution prepared with 0.2 N H2SO4 and filter-sterilized under 100% N2 gas atmosphere.
477 ACIDIHALOBACTER PROSPERUS MEDIUM