Main sol. 490
Internal Solution-ID: S996
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Yeast extract
(OXOID) |
2.0 | g | 2 | - | |
|
Trypticase peptone
(BD BBL) |
2.0 | g | 2 | - | |
| NaCl | 29.0 | g | 29 | 496.235 | |
| KCl | 1.5 | g | 1.5 | 20.121 | |
| NH4Cl | 1.0 | g | 1 | 18.695 | |
| K2HPO4 x 3 H2O | 0.4 | g | 0.4 | 2.297 | |
| MgCl2 x 6 H2O | 1.7 | g | 1.7 | 8.362 | |
|
2-Mercaptoethanesulfonic acid
(coenzyme M) |
0.5 | g | 0.5 | 3.516 | |
| Modified Wolin's mineral solution | 10.0 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| Na2CO3 | 2.5 | g | 2.5 | 23.588 | |
| Trimethylamine-HCl | 2.0 | g | 2 | - | |
| Na2S x 9 H2O | 0.3 | g | 0.3 | 1.249 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except carbonate, trimethylamine and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Distribute medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use add trimethylamine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of the complete medium to 8.5 with a sterile anoxic stock solution of Na2CO3 (5% w/v). | |||||
Media with this solution
| 490 | METHANOLOBUS OREGONENSIS MEDIUM |