MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 1223

Download this solution as

CSV JSON PDF

Internal Solution-ID: S2431

Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 75.0 g 75 1283.37
Na2CO3 3.0 g 3 28.305
NaHCO3 20.0 g 20 238.075
KCl 0.1 g 0.1 1.341
K2HPO4 0.3 g 0.3 1.722
MgCl2 x 6 H2O 0.5 g 0.5 2.459
CaCl2 x 2 H2O 0.2 g 0.2 1.36
NH4Cl 1.0 g 1 18.695
Na2S x 9 H2O 0.4 g 0.4 1.665
Yeast extract 0.5 g 0.5 -
Peptone 5.0 g 5 -
Cysteine-HCl 0.5 g 0.5 3.172
Modified Wolin's mineral solution II 10.0 ml - -
Resazurin 10.0 mg 0.01 0.044
Distilled water 1000.0 ml - -
1 Adjust to pH 9.0
2 Prepare the medium omitting Na2CO3, NaHCO3, Na2S, Cysteine-HCl, CaCl2 x 2 H2O and MgCl2 x 6 H2O. Boil the medium and cool under nitrogen, add the Na2CO3 and NaHCO3 and adjust the pH to 9.0 with 10 M KOH. Dispense into tubes or bottle fitted with rubber stoppers. Sterilize by autoclaving and add the Na2S (neutralized), Cysteine-HCl, CaCl2 x 2 H2O and MgCl2 x 6 H2O from sterile stock solutions.
1223 TINDALLIA TEXCOCOENSIS MEDIUM