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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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Current criteria: Bromocresol purple
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68 HALOTHIOBACILLUS NEAPOLITANUS MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
|
Agar
optional |
15 | 44.599 |
| Na2S2O3 x 5 H2O | 10 | 40.292 |
| KH2PO4 | 4 | 29.393 |
| K2HPO4 | 4 | 22.965 |
| MgSO4 x 7 H2O | 0.8 | 3.246 |
| NH4Cl | 0.4 | 7.478 |
| Na2-EDTA | 0.25 | 0.744 |
| ZnSO4 x 7 H2O | 0.11 | 0.383 |
| CaCl2 x 2 H2O | 0.028 | 0.188 |
| MnCl2 x 4 H2O | 0.025 | 0.128 |
| FeSO4 x 7 H2O | 0.025 | 0.09 |
| CoCl2 x 6 H2O | 0.008 | 0.062 |
| CuSO4 x 5 H2O | 0.008 | 0.031 |
| (NH4)6Mo7O24 x 4 H2O | 0.006 | - |
| Bromocresol purple | - | - |
| Show more | ||
Solutions:
- 5 ml Trace element solution (Vishniac & Santer, 1957)
333 THERMITHIOBACILLUS MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 10 | 29.733 |
| KH2PO4 | 4 | 29.393 |
| K2HPO4 | 4 | 22.965 |
| K2S4O6 | 3 | - |
| MgSO4 x 7 H2O | 0.8 | 3.246 |
| Na2-EDTA | 0.5 | 1.487 |
| NH4Cl | 0.4 | 7.478 |
| NaOH | 0.11 | 2.75 |
| ZnSO4 x 7 H2O | 0.11 | 0.383 |
| CaCl2 x 2 H2O | 0.073 | 0.499 |
| FeSO4 x 7 H2O | 0.05 | 0.18 |
| MnCl2 x 4 H2O | 0.025 | 0.126 |
| CoCl2 x 6 H2O | 0.005 | 0.039 |
| (NH4)6Mo7O24 x 4 H2O | 0.005 | - |
| CuSO4 x 5 H2O | 0.002 | 0.008 |
| Distilled water | - | - |
| Bromocresol purple | - | - |
Solutions:
- 10 ml Trace element solution
486 THIOBACILLUS THIOPARUS (TK-m) MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Na2S2O3 x 5 H2O | 5 | 20.146 |
| KH2PO4 | 2 | 14.697 |
| K2HPO4 | 2 | 11.483 |
| NH4Cl | 0.4 | 7.478 |
| Na2CO3 | 0.4 | 3.774 |
| MgCl2 x 6 H2O | 0.2 | 0.984 |
| Na2-EDTA | 0.05 | 0.149 |
| NaOH | 0.011 | 0.275 |
| ZnSO4 x 7 H2O | 0.011 | 0.038 |
| CaCl2 x 2 H2O | 0.007 | 0.05 |
| FeSO4 x 7 H2O | 0.005 | 0.018 |
| MnCl2 x 4 H2O | 0.003 | 0.013 |
| CoCl2 x 6 H2O | 5.0e-4 | 0.004 |
| (NH4)6Mo7O24 x 4 H2O | 5.0e-4 | - |
| CuSO4 x 5 H2O | 2.0e-4 | 8.0e-4 |
| Nicotinic acid | 6.0e-5 | 4.9e-4 |
| Pyridoxine hydrochloride | 6.0e-5 | 2.9e-4 |
| Riboflavin | 6.0e-5 | 1.6e-4 |
| Calcium pantothenate | 6.0e-5 | 1.3e-4 |
| p-Aminobenzoic acid | 3.0e-5 | 2.2e-4 |
| Thiamine-HCl x 2 H2O | 3.0e-5 | 8.0e-5 |
| Biotin | 3.0e-6 | 1.2e-5 |
| Vitamin B12 | 3.0e-6 | 2.2e-6 |
| Bromocresol purple | - | - |
| Show more | ||
Solutions:
- 3 ml Vitamin solution
- 1 ml Trace element solution