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Search for media using compounds and concentrations.

The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Haemin

Your search received 41 results.

78a CHOPPED MEAT MEDIUM FOR TREPONEMA SP.

Compound Conc. [g/L] Conc. [mM]
Ground beef 473.934 -
Casitone 28.436 -
Agar 14.218 42.274
K2HPO4 4.739 27.21
Yeast extract 4.739 -
L-Glutamine 0.664 4.54
L-Histidine 0.569 3.666
L-Serine 0.474 4.51
L-Cysteine HCl 0.474 3.007
Ethanol 0.18 3.909
Vitamin K3 0.047 -
Hemin 0.005 0.007
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
  • 5 ml Vitamin solution
  • 50 ml Amino acid solution (filter sterilized)

78c CHOPPED MEAT MEDIUM FOR FRETIBACTERIUM SP.

Compound Conc. [g/L] Conc. [mM]
Ground beef 586.166 -
Casein peptone 35.17 -
Na-formate 7.034 103.43
Na-fumarate 7.034 43.953
K2HPO4 5.862 33.654
Yeast extract 5.862 -
Ethanol 2.227 48.349
L-Cysteine HCl x H2O 0.586 3.337
DL-Dithiothreitol 0.352 2.279
Haemin 0.003 0.004
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Solutions:
  • 50 ml Clarified rumen fluid
  • 20 ml Fatty acid mixture
  • 1 ml Wolin's vitamin solution (10x)
  • 5 ml Haemin solution
  • 2 ml Vitamin K1 solution
Gas phase:
  • 80 % N2
  • 20 % CO2

78d MEDIUM FOR TREPONEMA PARVUM

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
K2HPO4 5 28.707
Glutathione 1.4 4.556
Na-pyruvate 0.5 4.544
L-Cysteine HCl 0.5 3.172
N-Acetylglucosamine 0.5 2.26
Ethanol 0.19 4.124
Casamino acids 0.16 -
Yeast extract 0.16 -
Tryptone peptone 0.16 -
Ascorbic acid 0.1 0.568
L-Glutamine 0.07 0.479
L-Histidin 0.06 0.387
L-Serin 0.05 0.476
Vitamin K3 0.05 -
Resazurin 1.0e-3 0.004
Hemin 5.0e-4 7.7e-4
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
  • 5 ml Vitamin solution
  • 5 ml Amino acid solution
  • 5 ml Peptone mixture
  • 5 ml Hemin solution
  • 5 ml Ascorbic acid solution 2%(w/v)
  • 5 ml N-acetylglucosamin- and pyruvate solution
  • 10 ml Glutathione solution 14%(w/v)
  • 10 ml Sugar solution
Gas phase:
  • 80 % N2
  • 20 % CO2

104 PYG MEDIUM (modified)

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
Glucose 5 27.753
Peptone 5 -
Trypticase peptone 5 -
Beef extract 5 -
L-Cysteine HCl x H2O 0.5 -
NaHCO3 0.4 4.762
NaCl 0.08 1.369
KH2PO4 0.04 0.294
K2HPO4 0.04 0.23
MgSO4 x 7 H2O 0.02 0.081
CaCl2 x 2 H2O 0.01 0.068
Hemin 0.005 0.008
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Solutions:
  • 40 ml Salt solution
  • 0.2 ml Vitamin K1 solution
  • 10 ml Haemin solution

110 CHOPPED MEAT MEDIUM WITH CARBOHYDRATES

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
K2HPO4 5 28.707
Yeast extract 5 -
D-Glucose 4 22.203
Maltose 1 2.921
Cellobiose 1 2.921
Starch 1 -
L-Cysteine HCl 0.5 3.172
Ethanol 0.19 4.124
Vitamin K3 0.05 -
Hemin 0.005 0.008
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
Gas phase:
  • 100 % N2

192 PILOBOLUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Na-acetate 10 121.901
Yeast extract 2 -
K2HPO4 1 5.741
(NH4)2SO4 0.66 4.995
MgSO4 x 7 H2O 0.5 2.029
Thiamine-HCl x 2 H2O 0.01 0.027
Haemin 0.01 0.015
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209 EUBACTERIUM LENTUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
K2HPO4 5 28.707
Arginine 5 28.703
Yeast extract 5 -
L-Cysteine HCl 0.5 3.172
Ethanol 0.19 4.124
Vitamin K3 0.05 -
Hemin 0.005 0.008
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution

228 PEPTOCOCCUS GLYCINOPHILUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
Trypticase peptone 5 -
Peptone 5 -
Beef extract 5 -
Glycine 3 39.963
L-Cysteine HCl x H2O 0.5 2.847
NaHCO3 0.4 4.762
NaCl 0.08 1.369
KH2PO4 0.04 0.294
K2HPO4 0.04 0.23
MgSO4 x 7 H2O 0.02 0.081
CaCl2 x 2 H2O 0.01 0.068
Hemin 0.005 0.008
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Solutions:
  • 40 ml Salt solution
  • 0.2 ml Vitamin K1 solution
  • 10 ml Haemin solution
Gas phase:
  • 80 % N2
  • 20 % CO2

328 PYG MEDIUM WITH VOLATILE FATTY ACIDS

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
Glucose 5 27.753
Beef extract 5 -
Trypticase 5 -
(NH4)2SO4 0.5 3.784
L-Cysteine HCl x H2O 0.5 2.847
NaHCO3 0.4 4.762
NaCl 0.08 1.369
KH2PO4 0.04 0.294
K2HPO4 0.04 0.23
MgSO4 x 7 H2O 0.019 0.078
CaCl2 x 2 H2O 0.012 0.082
Haemin 0.005 0.008
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Solutions:
  • 40 ml Mineral solution
  • 3.1 ml Fatty acid mixture
Gas phase:
  • 100 % N2

330 RUMEN BACTERIA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2CO3 4 37.74
Trypticase peptone 2 -
Glycerol 0.5 5.429
D-Glucose 0.5 2.775
Maltose 0.5 1.461
Cellobiose 0.5 1.461
Starch 0.5 -
Yeast extract 0.5 -
NaCl 0.456 7.803
K2HPO4 0.3 1.722
L-Cysteine HCl x H2O 0.25 1.423
Na2S x 9 H2O 0.25 1.041
(NH4)2SO4 0.228 1.725
KH2PO4 0.228 1.675
MgSO4 x 7 H2O 0.095 0.385
CaCl2 x 2 H2O 0.061 0.414
Hemin 1.0e-3 0.002
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Solutions:
  • 38 ml Mineral solution
  • 2 ml Haemin solution
  • 3.1 ml Volatile fatty acid mixture
Gas phase:
  • 100 % CO2
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