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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Na-formate

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78c CHOPPED MEAT MEDIUM FOR FRETIBACTERIUM SP.

Compound Conc. [g/L] Conc. [mM]
Ground beef 586.166 -
Casein peptone 35.17 -
Na-formate 7.034 103.43
Na-fumarate 7.034 43.953
K2HPO4 5.862 33.654
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Solutions:
  • 50 ml Clarified rumen fluid
  • 20 ml Fatty acid mixture
  • 1 ml Wolin's vitamin solution (10x)
  • 5 ml Haemin solution
  • 2 ml Vitamin K1 solution
Gas phase:
  • 80 % N2
  • 20 % CO2

119 METHANOBACTERIUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 3.988 47.473
Na-formate 1.994 29.321
Na-acetate 0.997 12.154
KH2PO4 0.499 3.663
L-Cysteine HCl x H2O 0.499 2.838
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Solutions:
  • 1 ml Trace element solution SL-10
  • 50 ml Sludge fluid
  • 20 ml Fatty acid mixture
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
Gas phase:
  • 80 % H2
  • 20 % CO2

119a METHANOBREVIBACTER MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 3.988 47.473
Na-formate 1.994 29.321
Na-acetate 0.997 12.154
Yeast extract 0.997 -
KH2PO4 0.499 3.663
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 200 ml Clarified rumen fluid
  • 20 ml Fatty acid mixture
Gas phase:
  • 80 % H2
  • 20 % CO2

119b METHANOMASSILIICOCCUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 7.888 246.191
Na-formate 1.992 29.292
Na-acetate 0.996 12.142
NaHCO3 0.996 11.856
KH2PO4 0.498 3.66
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 50 ml Sludge fluid
  • 20 ml Fatty acid mixture
Gas phase:
  • 66 % N2
  • 27 % H2
  • 7 % CO2

119c GUDONGELLA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na-formate 2 29.409
Na-acetate 1 12.19
NaHCO3 0.5 5.952
KH2PO4 0.5 3.674
L-Cysteine HCl x H2O 0.5 2.847
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 50 ml Sludge fluid
  • 20 ml Fatty acid mixture
Gas phase:
  • 100 % N2

157 WOLINELLA MEDIUM

Compound Conc. [g/L] Conc. [mM]
K2HPO4 5 28.707
Na2-fumarate 4 24.994
Na-formate 3 44.113
(NH4)2SO4 1 7.568
Yeast extract 1 -
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Gas phase:
  • 100 % N2

321 METHANOCORPUSCULUM AGGREGANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na-formate 5 73.522
Na2CO3 1.5 14.153
NH4Cl 1 18.695
Na-acetate 1 12.19
Trypticase peptone 1 -
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Solutions:
  • 50 ml Minerals solution
  • 10 ml Modified Wolin's mineral solution
  • 5 ml Clarified rumen fluid
  • 5 ml Sludge fluid
Gas phase:
  • 80 % H2
  • 20 % CO2

322 METHANOSPHAERA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 7.704 240.443
NaHCO3 3.891 46.318
KH2PO4 2.724 20.015
Trypticase peptone 1.946 -
Yeast extract 1.946 -
NH4Cl 0.973 18.186
NaCl 0.584 9.987
K2HPO4 0.584 3.351
Na-formate 0.486 7.152
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Solutions:
  • 100 ml Clarified rumen fluid
  • 10 ml Modified Wolin's mineral solution
  • 2 ml Wolin's vitamin solution (10x)
  • 3 ml FeSO4 x 7 H2O solution (0.1% w/v)
Gas phase:
  • 80 % H2
  • 20 % CO2

332 METHANOCULLEUS BOURGENSIS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na-formate 5 73.522
Na2CO3 1.5 14.153
NH4Cl 1 18.695
Na-acetate 1 12.19
Yeast extract 1 -
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Gas phase:
  • 80 % H2
  • 20 % CO2

337 SYNTROPHOCOCCUS SUCROMUTANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Lactose 4.96 14.491
Trypticase peptone 4.96 -
NaHCO3 2.48 29.523
Na-formate 0.595 8.753
KH2PO4 0.496 3.645
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Solutions:
  • 921 ml Solution A
  • 50 ml Solution B
  • 1 ml Solution C
  • 25 ml Solution D
  • 10 ml Solution E
  • 1 ml Solution F
  • 50 ml Mineral solution
  • 1 ml Trace element solution SL-10
  • 300 ml Clarified rumen fluid
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % N2
  • 20 % CO2
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