1000
1000 .
MJANHOX-NO3 MEDIUM WITH SUPPLEMENT
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1000 #
ml
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 3.000 | g | 3 | 51.335 | |
| K2HPO4 | 0.014 | g | 0.014 | 0.08 | |
| CaCl2 x 2 H2O | 0.014 | g | 0.014 | 0.095 | |
| NH4Cl | 0.040 | g | 0.04 | 0.748 | |
| MgSO4 x 7 H2O | 0.340 | g | 0.34 | 1.379 | |
| MgCl2 x 6 H2O | 0.418 | g | 0.418 | 2.056 | |
| KCl | 0.033 | g | 0.033 | 0.443 | |
| Fe2(SO4)3 x n H2O | 5.000 | mg | 0.005 | 0.013 | |
| NiCl2 x 6 H2O | 5.000 | µg | 5.0e-6 | 3.9e-5 | |
| Na2SeO3 x 5 H2O | 5.000 | µg | 5.0e-6 | 1.9e-5 | |
| Na2SiO3 x 9 H2O | 0.500 | g | 0.5 | 1.759 | |
| Trace mineral solution | 1.000 | ml | - | - | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Before autoclaving, the pH of the Medium is adjusted with NaOH to 7.5-8.0. After autoclaving, the filter-sterilized vitamin solution (0.1% volume), and each of the separately autoclaved, concentrated solutions are added to the medium at a final concentration described below. Then a mix gas (80% H2, 20% CO2) is purged for 5 min. Finally, the mix gas is compressed into gas phase (> 80% volume of the tube or bottle) at 3 atm. | |||||
| Na2S2O3 x 5 H2O | 10.000 | mM | 2.482 | 10 | |
| NaHCO3 | 0.200 | % | 2 | 23.808 | |
| NaNO3 | 0.050 | % | 0.5 | 5.883 | |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 |
| MnSO4 x 2 H2O | 0.50 | g | 0.5 | 2.673 |
| CoSO4 x 7 H2O | 0.50 | g | 0.5 | 3.226 |
| ZnSO4 x 7 H2O | 0.18 | g | 0.18 | 0.626 |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 |
| AlK(SO4)2 x 12 H2O | 0.02 | g | 0.02 | 0.042 |
| H3BO3 | 0.01 | g | 0.01 | 0.162 |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 7.9 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Gas composition
- 80 % H2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 3 | 51.335 |
| Na2S2O3 x 5 H2O | 2.482 | 10 |
| NaHCO3 | 2 | 23.808 |
| NaNO3 | 0.5 | 5.883 |
| Na2SiO3 x 9 H2O | 0.5 | 1.759 |
| Show more | ||
