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104a. ANAEROBACULUM MEDIUM

Culture medium recipe

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* Modified for Anaerobaculum hydrogeniformans DSM 22491
beta

Anaerobic

Equipment needed: Hungate tubes Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(BD BBL)
5.0 g 4.808 -
2
(pepsin-digested)
5.0 g 4.808 -
3 10.0 g 9.615 -
4 8.0 g 7.692 131.622
5 Salt solution 40.0 ml - -
6
(0.1% w/v)
0.5 ml 4.8e-4 0.002
7 0.5 g 0.481 2.737
8 1.0 g 0.962 5.337
9 2.5 g 2.404 9.686
10 1000.0 ml 959.814 53277.7
Instructions:
  1. Dissolve ingredients (except cysteine, glucose and thiosulfate), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add glucose and thiosulfate from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 7.0, if necessary.
Solution occurs in 6 other media (first defined in medium 104 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.25 g 0.25 1.701
2 0.50 g 0.5 2.029
3 1.00 g 1 5.741
4 1.00 g 1 7.348
5 10.00 g 10 119.038
7 1000.00 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Hungate tubes

Associated strains

All strains for this medium: 3
Strains with modifications: 3
Anaerobaculum hydrogeniformans DSM 22491

Gas composition

  • 100 % N2

Light

Intensity lux

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Yeast extract 9.615 -
NaCl 7.692 131.622
Trypticase peptone 4.808 -
Peptone from meat 4.808 -
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Cultivation metadata from 3 strains BacDive

halophily not 1 strain
halophily moderate 1 strain
pH 8 - 10 2 strains
pH 6 - 8 2 strains
pH 4 - 6 2 strains
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Similar media

104c. PY + X MEDIUM (N2/CO2) 75 %
152. NICOTINIC ACID MEDIUM 71 %
104b. PY + X MEDIUM 69 %
169. TREPONEMA ZUELZERAE MEDIUM 69 %
104d. MALEDIVIBACTER MEDIUM (BETAINE) 65 %

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