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1059. GEOALKALIBACTER MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.499 9.329
2 0.2 g 0.2 2.677
3 0.1 g 0.1 0.491
4 0.2 g 0.2 1.467
5 1.0 g 0.998 17.077
6 Trace element solution SL-10 1.0 ml - -
7 Selenite-tungstate solution 1.0 ml - -
8 3.0 g 2.994 28.249
9 10.0 g 9.98 118.801
10
(powdered)
10.0 g 9.98 311.234
11 0.1 g 0.1 -
12 2.5 g 2.495 30.414
13 1000.0 ml 996.215 55298.2
Instructions:
  1. Dissolve ingredients (ecxept carbonate, hydrogencarbonate, sulfur, yeast extract and acetate), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add solid carbonate and bicarbonate, adjust pH to 9.0 - 9.2, and dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur. Sterilize medium by autoclaving at 110°C (!) for 20 min. Add yeast extract and acetate from sterile anoxic stock solutions prepared under 100% N2 gas.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.1
Equipment needed: Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.197 55408.2
Sulfur 9.98 311.234
NaHCO3 9.98 118.801
Na2CO3 2.994 28.249
Sodium acetate 2.495 30.414
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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