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DSMZ

DSMZ 1063 . MICROCELLA ALKALIPHILA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1063 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase soy broth 30 g 30 -
Yeast extract 3 g 3 -
Distilled water 944 ml - -
Mineral solution 50 ml - -
Seven vitamins solution 5 ml - -
Trace element solution SL-10 1 ml - -
Na-sesquicarbonate solution 1 M - -
1 After sterilization add sterile 1M Na-sesquicarbonate solution to achieve the pH of 9.5 (see medium 31).

Mineral solution #

1000
Solution occurs in 4 other media (first defined in Medium 335).
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 10.0 g 10 73.484
MgCl2 x 6 H2O 6.6 g 6.6 32.464
NaCl 8.0 g 8 136.893
NH4Cl 8.0 g 8 149.561
CaCl2 x 2 H2O 1.0 g 1 6.802
Distilled water 1000.0 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Seven vitamins solution #

1000
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Na-sesquicarbonate solution #

100
Solution occurs in 1 other media (first defined in Medium 31).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 4.2 g 42 499.958
Na2CO3 anhydrous 5.3 g 53 500.057
Distilled water 100.0 ml - -

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Trypticase soy broth 30 -
Yeast extract 3 -
KH2PO4 0.5 3.674
NH4Cl 0.4 7.478
NaCl 0.4 6.845
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen obligate aerobe 1 strain
temperature mesophilic 1 strain