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1076b. SP4-Z MEDIUM

Culture medium recipe

Vol.
×
  • The difference between this medium and "normal" SP4 medium (Medium 1076) is that the yeast extract was left out and DNA was added instead. Since most mycoplasmas use either glucose or arginine as their energy source one is added to the media. Do not combine both in one medium, arginine can inhibit the growth of some glucose utilizers (unknown reason).
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.70 g 3.535 -
2 2.00 g 10.101 -
3
(BD)
1.00 g 5.051 -
4 , adjust if required 130.00 ml 655.388 36379.5
5
(fish sperm; SERVA)
0.04 g 0.202 -
6
(10x concentrated; GIBCO)
10.00 ml - -
7
(BD; 2%, autoclaved)
20.00 ml - -
8
(heat-inactivated)
17.00 ml - -
9
(heat-inactivated)
17.00 ml - -
10 , either
(50% aqueous solution)
2.00 ml - -
11 , or
(50% aqueous solution)
2.00 ml - -
12
optional
0.40 g 2.02 33.639
Instructions:
  1. For 200 ml of medium add: #1-5
  2. Adjust pH to 7.4; autoclave at 121°C for 15 minutes.
  3. Add aseptically: #6-9
  4. As energy source add either #10 or #11
  5. For Ureaplasma add #12 instead of Glucose or Arginine
  6. For agar plates use only 126 ml of distilled water and add 2 g of agar . Medium containing agar should be placed at 55°C before adding supplements.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.4
Equipment needed: Autoclave

Associated strains

All strains for this medium: 98
Strains with modifications: 98
1076 SP4 MEDIUM
a SP4 MEDIUM WITH GLUCOSE
b SP4-Z MEDIUM

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 655.388 36379.5
Tryptone 10.101 -
Bacto-Peptone 5.051 -
PPLO broth 3.535 -
Urea
optional
2.02 33.639
DNA 0.202 -
CMRL-1066 medium - -
Yeastolate - -
Fetal bovine serum - -
Swine serum - -
Arginine HCl - -
Glucose - -

Cultivation metadata from 98 strains BacDive

pH 8 - 10 1 strain
pH 6 - 8 1 strain
pH 4 - 6 1 strain
phyla 3 97 strains
oxygen microaerophile 28 strains
oxygen aerobe 8 strains
oxygen facultative anaerobe 1 strain
oxygen facultative aerobe 1 strain
temperature mesophilic 96 strains
temperature thermophilic 1 strain
temperature psychrophilic 1 strain

Similar media

680. TRYPTONE YEAST EXTRACT AGAR (TYG) 59 %
J140. TRYPTONE-YEAST EXTRACT AGAR 59 %
1207. TGE MEDIUM 58 %
J821. TRYPTONE-YEAST EXTRACT (ISP-1) AGAR 57 %
893. TS AGAR 56 %

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