DSMZ 1104 . DETHIOBACTER MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Water bath Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 6.00 g 5.923 101.352
K2HPO4 0.50 g 0.494 2.834
NaHCO3 8.00 g 7.897 94.008
Na2CO3 22.00 g 21.718 204.907
NH4Cl 0.20 g 0.197 3.691
MgCl2 x 6 H2O 0.20 g 0.197 0.971
Trace elements solution (Pfennig & Lippert,1966) 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Yeast extract 0.05 g 0.049 -
Wolin's vitamin solution 10.00 ml - -
Ethanol absolute 0.60 ml - -
Na-acetate 0.40 g 0.395 4.813
Na2S2O3 x 5 H2O 2.50 g 2.468 9.944
Sulfur
(powdered)
1.50 g 1.481 46.172
Na2S x 9 H2O 0.24 g 0.237 0.986
Distilled water 1000.00 ml - -
1 Dissolve sodium chloride and hydrogenphosphate, then sparge solution with 100% N2 gas for 30 -45 min to make it anoxic. Add and dissolve carbonates, then dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur. Sterilize medium by heating cultivation vessels in a water bath to 90 - 100°C for 1- 2 hours on each of 3 successive days. After sterilization add ammonium chloride, magnesium chloride, trace elements, yeast extract, vitamins, ethanol, acetate, thiosulfate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The stock solutions of thiosulfate and vitamins should be sterilized by filtration. Adjust pH of the complete medium to 9.5. After inoculation add sterile 100% H2 gas to 0.5 bar overpressure.
Solution occurs in 27 other media (first defined in Medium 1369).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.20 g 2.2 7.913
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.03 g 0.03 0.485
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.03 g 0.03 0.176
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 pH 3.0-4.0
Solution occurs in 174 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 12.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.5
Equipment needed: Filter, Gassing station, Hungate tubes, Water bath
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Gas composition

  • 66 % N2
  • 33 % H2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 21.718 204.907
NaHCO3 7.897 94.008
NaCl 5.923 101.352
Na2S2O3 x 5 H2O 2.468 9.944
Sulfur 1.481 46.172
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Cultivation metadata from 2 strains BacDive

phyla 2 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains