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1104. DETHIOBACTER MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 6.00 g 5.923 101.347
2 0.50 g 0.494 2.834
3 8.00 g 7.897 94.009
4 22.00 g 21.718 204.907
5 0.20 g 0.197 3.691
6 0.20 g 0.197 0.971
7 Trace elements solution (Pfennig & Lippert,1966) 1.00 ml - -
8 Selenite-tungstate solution 1.00 ml - -
9 0.05 g 0.049 -
10 Vitamin solution 10.00 ml - -
11 0.60 ml 0.468 10.157
12 0.40 g 0.395 4.813
13 2.50 g 2.468 9.944
14
(powdered)
1.50 g 1.481 46.178
15 0.24 g 0.237 0.986
16 1000.00 ml 985.397 54697.8
17 , might need strain adjustment 1.10 g/l - -
Instructions:
  1. Dissolve sodium chloride and hydrogenphosphate, then sparge solution with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonates, then dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur. Sterilize medium by heating cultivation vessels in a water bath to 90 - 100°C for 1- 2 hours on each of 3 successive days. After sterilization add ammonium chloride, magnesium chloride, trace elements, yeast extract, vitamins, ethanol, acetate, thiosulfate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The stock solutions of thiosulfate and vitamins should be sterilized by filtration. Adjust pH of the complete medium to 9.5. After inoculation add sterile 100% H2 gas to 0.5 bar overpressure.
Solution occurs in 23 other media (first defined in medium 1369 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.00 g 5 17.228
2 2.20 g 2.2 7.913
3 0.10 g 0.1 0.348
4 0.03 g 0.03 0.152
5 0.03 g 0.03 0.485
6 0.20 g 0.2 0.841
7 0.03 g 0.03 0.176
8 0.03 g 0.03 0.126
9 0.03 g 0.03 0.124
10 1000.00 ml 998.207 55408.8
Instructions:
  1. pH 3.0-4.0
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.5
Equipment needed: Filter, Hungate tubes

Associated strains

All strains for this medium: 2
Strains with modifications: 2

Gas composition

  • 100 % H2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.222 55354.2
Na2CO3 21.718 204.907
NaHCO3 7.897 94.009
NaCl 5.923 101.347
Na2S2O3 x 5 H2O 2.468 9.944
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Cultivation metadata from 2 strains BacDive

phyla 2 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains

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