DSMZ 110 . CHOPPED MEAT MEDIUM WITH CARBOHYDRATES

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Culture medium recipe

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Selected strain: Clostridium perfringens DSM 756

This strain uses the standard medium without modifications.

Equipment needed: Autoclave Hungate tubes Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ground beef
(fat free)
500.0 g 500 -
Distilled water 1000.0 ml - -
NaOH
(1 N)
25.0 ml - -
1 Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1000 ml, and then add:
Casitone 30.0 g 30 -
Yeast extract 5.0 g 5 -
K2HPO4 5.0 g 5 28.707
Sodium resazurin
(0.1% w/v)
0.5 ml 5.0e-4 -
D-Glucose 4.0 g 4 22.203
Cellobiose 1.0 g 1 2.921
Maltose 1.0 g 1 2.921
Starch
(soluble)
1.0 g 1 -
L-Cysteine HCl , add to make medium anoxic 0.5 g 0.5 3.172
Agar , if required 15.0 g 15 44.599
2 In some cases (as indicated in the catalogue) the addition of Haemin and Vitamin K1 or Vitamin K3 is necessary. Add to 1000 ml of medium after autoclaving: and either or
Haemin solution 10.0 ml - -
Vitamin K1 solution , alternative 10.0 ml - -
Vitamin K3 solution 10.0 ml - -
3 To make medium anoxic boil it, cool under 100% N2 gas atmosphere, add 0.5 g/l L-cysteine hydrochloride and adjust pH to 7.0. Dispense under same gas atmosphere 7 ml medium into Hungate-type tubes (for strains demanding meat particles put those first into the tube (use 1 part meat particles to 4 or 5 parts fluid)). Autoclave at 121°C for 30 min. For agar slants use 15.0 g agar per 1000.0 ml medium.
Solution occurs in 15 other media (first defined in Medium 78).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Haemin 50 mg 0.5 0.767
NaOH
(1 N)
1 ml - -
Distilled water 100 ml - -
1 Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated.
Solution occurs in 12 other media (first defined in Medium 78).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin K1 0.1 ml - -
Ethanol
(95 %)
20.0 ml 950 20620.8
1 Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle.
Solution occurs in 7 other media (first defined in Medium ).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin K3 5 mg 0.05 -
Ethanol
(95%)
1 ml 9.5 206.208
Water 100 ml - -
1 Dissolve 5 mg vitamin K3 in 1 ml 95% ethanol, dilute to 0.05 mg/ml in water and filter sterilize. Store refrigerated in a brown bottle.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 232
Strains with modifications: 30
Clostridium perfringens DSM 756

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
K2HPO4 5 28.707
Yeast extract 5 -
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Cultivation metadata from 232 strains BacDive

halophily non-halophilic 2 strains
pH 6 - 8 12 strains
pH 8 - 10 6 strains
pH 4 - 6 6 strains
phyla 8 232 strains
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