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110a. CMC MEDIUM (N2/CO2)

Culture medium recipe

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* Modified for unclassified bacterium DSM 102154
beta

Anaerobic

Equipment needed: Hungate tubes Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 30.0 g 30 -
2 5.0 g 5 -
3 5.0 g 5 28.707
4
(0.1% w/v)
0.5 ml 5.0e-4 0.002
5 0.5 g 0.5 2.847
6 4.0 g 4 22.203
7 1.0 g 1 2.921
8 1.0 g 1 2.921
9
(soluble)
1.0 g 1 -
10 1.5 g 1.5 14.153
11 Meat filtrate 1000.0 ml - -
Instructions:
  1. Dissolve ingredients, except cysteine, carbohydrates and carbonate, boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere, add 0.5 g/l L-cysteine- HCl x H2O and dispense under same gas atmosphere into Hungate-type tubes (for strains demanding meat particles put those first into the tube, use 1 part meat particles to 4 or 5 parts fluid). Autoclave at 121°C for 20 min. After autoclaving add glucose, cellobiose, maltose and starch from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of medium to pH 7, if necessary.
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(fat free)
500 g 487.805 -
2
(1 N)
25 ml 51.951 1298.87
3 1000 ml 973.86 54057.4
Instructions:
  1. Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 46
Strains with modifications: 46
unclassified bacterium DSM 102154

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 973.86 54057.4
Ground beef 487.805 -
NaOH 51.951 1298.87
Casitone 30 -
K2HPO4 5 28.707
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Cultivation metadata from 46 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 2 33 strains
oxygen anaerobe 11 strains
oxygen aerobe 1 strain
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