Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Medium 11 | 990 | ml | - | - | |
Vitamin solution | 10 | ml | - | - | |
1 Prepare medium 11 with additional vitamins. Adjust pH to 6.2 - 6.5 |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Casein peptone
(tryptic digest) |
10.00 | g | 10 | - | |
Meat extract | 10.00 | g | 10 | - | |
Yeast extract | 5.00 | g | 5 | - | |
Glucose | 20.00 | g | 20 | 111.012 | |
Tween 80 | 1.00 | g | 1 | 0.764 | |
K2HPO4 | 2.00 | g | 2 | 11.483 | |
Na-acetate | 5.00 | g | 5 | 60.951 | |
(NH4)3 citrate | 2.00 | g | 2 | 8.223 | |
MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
MnSO4 x H2O | 0.05 | g | 0.05 | 0.296 | |
Distilled water | 1000.00 | ml | - | - | |
1 Adjust pH to 6.2 - 6.5. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.00 | mg | 0.002 | 0.008 |
Folic acid | 2.00 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.00 | mg | 0.01 | 0.049 |
Thiamine-HCl x 2 H2O | 5.00 | mg | 0.005 | 0.013 |
Riboflavin | 5.00 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.00 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.00 | mg | 0.005 | 0.01 |
p-Aminobenzoic acid | 1.00 | mg | 1.0e-3 | 0.007 |
Vitamin B12 | 0.01 | mg | 1.0e-5 | 7.4e-6 |
Distilled water | 1000.00 | ml | - | - |
Last modified: | 10.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Autoclave |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Glucose | 19.8 | 109.902 |
Meat extract | 9.9 | - |
Casein peptone | 9.9 | - |
Na-acetate | 4.95 | 60.341 |
Yeast extract | 4.95 | - |
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