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DSMZ 11b . MRS MEDIUM (pre-reduced)

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone
(tryptic digest)
10.00 g 10 -
Meat extract 10.00 g 10 -
Yeast extract 5.00 g 5 -
Glucose 20.00 g 20 111.012
Tween 80 1.00 g 1 0.764
Resazurin 1.00 mg 1.0e-3 0.004
K2HPO4 2.00 g 2 11.483
Na-acetate 5.00 g 5 60.951
(NH4)3 citrate 2.00 g 2 8.223
MgSO4 x 7 H2O 0.20 g 0.2 0.811
MnSO4 x H2O 0.05 g 0.05 0.296
Distilled water 1000.00 ml - -
L-Cysteine HCl x H2O 0.50 g 0.5 2.847
1 Cysteine is added after the medium has been boiled and cooled under CO2. Adjust pH to 6.2 - 6.5, distribute under N2 and autoclave.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.2 - 6.5
Equipment needed: Autoclave, Gassing station, Hungate tubes

Associated strains

All strains for this medium: 20
Strains with modifications: 8

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glucose 20 111.012
Casein peptone 10 -
Meat extract 10 -
Na-acetate 5 60.951
Yeast extract 5 -
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Cultivation metadata from 20 strains BacDive

pH 6 - 8 3 strains
phyla 1 12 strains
oxygen anaerobe 10 strains
oxygen microaerophile 3 strains
oxygen facultative anaerobe 1 strain
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