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1304. SULFURITALEA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Anaerobic workbench Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.2 g 0.199 0.981
2 0.1 g 0.1 0.678
3 1.7 g 1.695 19.941
4 0.1 g 0.1 0.755
5 0.1 g 0.1 0.733
6 0.1 g 0.1 1.337
7 Trace element solution SL-10 1.0 ml - -
8 Selenite-tungstate solution 1.0 ml - -
9 0.4 g 0.399 1.607
10 5.0 g 4.985 59.341
11 Vitamin solution 1.0 ml - -
12 1000.0 ml 995.221 55243.1
Instructions:
  1. Dissolve ingredients except trace elements, thiosulfate, bicarbonate and vitamins, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to remove dissolved oxygen and to saturate the solution with CO2. Dispense solution under 80% N2 and 20% CO2 gas atmosphere in vials suitable for anaerobic cultures (e.g., Balch-type tubes) to 30% of volume. Close vials with butyl rubber septa and autoclave. Add trace element solution, selenite-tungstate solution and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. The vitamin solution should be sterilized by filtration. Adjust pH of the complete medium to 6.7 - 7.0, if necessary. After inoculation, add 1 bar overpressure of sterile 100% H2 gas.
  2. Note: Use at least 10%(v/v) as inoculum.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.9
Equipment needed: Anaerobic workbench, Autoclave, Filter

Associated strains

All strains for this medium: 3
Strains with modifications: 3

Gas composition

  • 100 % H2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.197 55408.3
NaHCO3 4.985 59.341
NaNO3 1.695 19.941
Na2S2O3 x 5 H2O 0.399 1.607
MgCl2 x 6 H2O 0.199 0.981
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Cultivation metadata from 3 strains BacDive

halophily not 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 3 strains
oxygen anaerobe 2 strains
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