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1596. METHANOCALCULUS ALKALIPHILUS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.00 g 2.964 50.724
2 1.00 g 0.988 5.673
3 20.00 g 19.763 235.253
4 15.00 g 14.822 139.847
5 0.20 g 0.198 3.695
6 0.50 g 0.494 2.005
7 Trace elements solution (Pfennig & Lippert,1966) 1.00 ml - -
8 Selenite-tungstate solution 1.00 ml - -
9 3.40 g 3.36 49.402
10 0.16 g 0.158 1.927
11
(OXOID)
0.02 g 0.02 -
12 Vitamin solution 10.00 ml - -
13 0.25 g 0.247 1.029
14 1000.00 ml 986.371 54751.8
15 , may stimulate growth of some strains 20.00 mg 0.02 -
Instructions:
  1. Dissolve sodium chloride and hydrogenphosphate, then sparge solution with 100% N2 gas for at least 30 - 45 min to make it anoxic. Add and dissolve hydrogencarbonate and carbonate while gassing the head space only, then dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add ammonium chloride, magnesium sulfate, trace elements, formate, acetate, yeast extract, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration. The pH of the complete medium should be 9.5.
  2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5%(w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum.
Solution occurs in 23 other media (first defined in medium 1369 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.00 g 5 17.228
2 2.20 g 2.2 7.913
3 0.10 g 0.1 0.348
4 0.03 g 0.03 0.152
5 0.03 g 0.03 0.485
6 0.20 g 0.2 0.841
7 0.03 g 0.03 0.176
8 0.03 g 0.03 0.126
9 0.03 g 0.03 0.124
10 1000.00 ml 998.207 55408.8
Instructions:
  1. pH 3.0-4.0
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 9.5
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaHCO3 19.763 235.253
Na2CO3 14.822 139.847
Sodium formate 3.36 49.402
NaCl 2.964 50.724
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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