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1599. CONTUBERNALIS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.499 9.329
2 0.2 g 0.2 1.467
3 0.1 g 0.1 0.491
4 0.2 g 0.2 2.677
5 4.2 g 4.192 71.722
6 3.0 g 2.994 21.078
7 0.2 g 0.2 -
8 Trace element solution SL-10 1.0 ml - -
9 Selenite-tungstate solution 1.0 ml - -
10
(0.1% w/v)
0.5 ml 5.0e-4 0.002
11 3.3 g 3.293 39.204
12 6.5 g 6.487 -
13 3.0 g 2.994 36.497
14 0.5 g 0.499 2.078
15 1000.0 ml 996.215 55298.2
Instructions:
  1. Dissolve ingredients (except carbonates, acetate and sulfide) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Then, add and dissolve bicarbonate and carbonate, adjust pH to 9.5, dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add acetate and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas atmosphere. Adjust pH of complete medium to 9.5 - 9.7, if necessary.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.6
Equipment needed: Autoclave, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.197 55408.2
NaCO3 6.487 -
NaCl 4.192 71.722
NaHCO3 3.293 39.204
Sodium acetate 2.994 36.497
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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