DSMZ 1599 . CONTUBERNALIS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.5 g 0.5 9.348
KH2PO4 0.2 g 0.2 1.47
MgCl2 x 6 H2O 0.1 g 0.1 0.492
KCl 0.2 g 0.2 2.683
NaCl 4.2 g 4.2 71.869
Na2SO4 3.0 g 3 21.121
Yeast extract 0.2 g 0.2 -
Trace element solution SL-10 1.0 ml - -
Selenite-tungstate solution 1.0 ml - -
Sodium resazurin
(0.1% w/v)
0.5 ml 5.0e-4 -
NaHCO3 3.3 g 3.3 39.282
NaCO3 6.5 g 6.5 -
Na-acetate 3.0 g 3 36.57
Na2S x 9 H2O 0.5 g 0.5 2.082
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except carbonates, acetate and sulfide) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Then, add and dissolve bicarbonate and carbonate, adjust pH to 9.5, dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add acetate and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas atmosphere. Adjust pH of complete medium to 9.5 - 9.7, if necessary.
Solution occurs in 262 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 173 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Metadata

Last modified: 11.05.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.5 - 9.7
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCO3 6.5 -
NaCl 4.2 71.869
NaHCO3 3.3 39.282
Na-acetate 3 36.57
Na2SO4 3 21.121
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain