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DSMZ

DSMZ 1685 . HALORUSSUS VARIUS MEDIUM (MSC 15.2)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1685 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 210.210 g 210.21 3597.02
MgCl2 x 6 H2O 23.400 g 23.4 115.099
CaCl2 x H2O 21.700 g 21.7 195.525
KNO3 18.700 g 18.7 184.96
MgSO4 x 6 H2O 4.490 g 4.49 37.302
CaCO3 0.015 g 0.015 0.15
KBr 2.600 g 2.6 21.849
NaNO3 9.440 g 9.44 111.065
SrCl2 x 6 H2O 0.630 g 0.63 2.363
NH4Cl 0.050 g 0.05 0.935
KH2PO4 0.010 g 0.01 0.073
Glucose 1.000 g 1 5.551
Yeast extract
(BD Chemicals) 		0.100 g 0.1 -
FeEDTA solution 1.000 ml - -
Trace element solution 1.000 ml - -
Agar 20.000 g 20 59.465
Distilled water 1000.000 ml - -
1 Adjust the pH 6.9

FeEDTA solution #

1000
Solution occurs in 1 other media (first defined in Medium 1493).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 1.54 g 1.54 5.539
Na-EDTA 2.06 g 2.06 6.127
Distilled water 1000.00 ml - -

Trace element solution #

1000
Solution occurs in 1 other media (first defined in Medium 1493).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g 1.5 7.848
Fe(NH4)2(SO4)2 x 6 H2O 0.200 g 0.2 -
Na2SeO4 x 10 H2O 0.440 g 0.44 -
CoCl2 x 6 H2O 0.180 g 0.18 1.386
MnSO4 x H2O 0.120 g 0.12 0.71
Na2MoO4 0.160 g 0.16 0.777
Na2WO4 0.100 g 0.1 0.34
ZnSO4 x 7 H2O 0.540 g 0.54 1.878
AlCl3 0.040 g 0.04 0.3
NiCl2 x 6 H2O 0.025 g 0.025 0.193
H3BO3 0.110 g 0.11 1.779
CuSO4 0.210 g 0.21 1.316
MnCl2 0.190 g 0.19 1.51
Distilled water 1000.000 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals.
2 Final pH 7.0 (with KOH).

Metadata

Last modified: 22.02.22
Source: DSMZ
Medium type: Complex medium
Final pH: 6.9
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 210.21 3597.02
MgCl2 x 6 H2O 23.4 115.099
CaCl2 x H2O 21.7 195.525
Agar 20 59.465
KNO3 18.7 184.96
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