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DSMZ

DSMZ 215c . BHI MEDIUM FOR STRICT ANAEROBES

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Enterocloster lavalensis DSM 19851

* Modification: Supplement medium with 10.0 ml/l haemin and 0.2 ml/l vitamin K1 solution (see medium 78).

Growth conditions: anaerobic 35 °C

Main sol. 215c #

ml
Equipment needed: Gassing station Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Brain heart infusion
(DIFCO) 		37.00 g 37 -
L-Cysteine HCl x H2O 0.25 g 0.25 1.423
Na2S x 9 H2O 0.25 g 0.25 1.041
Vitamin K1 solution* 0.20 ml - -
Haemin solution* 10.00 ml - -
Distilled water 1000.00 ml - -
1 Prepare medium anoxically under 100% N2 gas atmosphere. Reducing agents are added after autoclaving from sterile anoxic solutions prepared under N2.

Vitamin K1 solution * #

20
Solution occurs in 12 other media (first defined in Medium 78).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin K1 0.1 ml - -
Ethanol
(95 %) 		20.0 ml 950 20620.8
1 Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle.

Haemin solution * #

100
Solution occurs in 15 other media (first defined in Medium 78).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Haemin 50 mg 0.5 0.767
NaOH
(1 N) 		1 ml - -
Distilled water 100 ml - -
1 Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated.

Metadata

Last modified: 18.01.24
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2 - 7.6
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 7
Strains with modifications: 3
Enterocloster lavalensis DSM 19851

+  0.20 ml Vitamin K1 solution
+  10.00 ml Haemin solution

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Brain heart infusion 37 -
L-Cysteine HCl x H2O 0.25 1.423
Na2S x 9 H2O 0.25 1.041
Distilled water - -
Haemin - -
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Cultivation metadata from 7 strains BacDive

phyla 2 7 strains
oxygen anaerobe 6 strains
temperature mesophilic 7 strains