DSMZ 535b . REACTIVATION WITH LIQUID MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Medium 535 , alternative 1000 ml - -
545. TRYPTONE SOYA BROTH (TSB) , alternative 1000 ml - -
1 Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 545. Subsequent subculturing may be carried out in liquid medium 545 or on agar plates.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone 17.0 g 17 -
Soy peptone 3.0 g 3 -
D(+)-Glucose 2.5 g 2.5 13.877
NaCl 5.0 g 5 85.558
K2HPO4 2.5 g 2.5 14.353
Agar 15.0 g 15 44.599
Distilled water 1000.0 ml - -
1 Adjust pH to 7.3.
2 Medium 545 is the same as Caso Bouillon (Merck or Oxoid, Germany).
Solution occurs in 18 other media (first defined in Medium 535).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase soy broth
(BBL 11768, Oxoid CM129 or Merck 5459)
30 g - -
Agar 20 g - -
Distilled water 1000 ml - -
1 pH 7.3
2 Autoclave at 121°C for 15 min.

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.3
Equipment needed: Autoclave
Edit in Medium builder

Associated strains

All strains for this medium: 58
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Trypticase soy broth 30 -
Casein peptone 17 -
Agar 15 44.599
NaCl 5 85.558
Soy peptone 3 -
Show more

Cultivation metadata from 58 strains BacDive

pH 6 - 8 8 strains
pH 8 - 10 7 strains
pH 4 - 6 5 strains
phyla 1 59 strains
oxygen aerobe 21 strains
Show more