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DSMZ

DSMZ 55 . FRUCTOSE MINERAL MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Cupriavidus necator DSM 422

This strain uses the standard medium without modifications.

Growth conditions: 30 °C

Main sol. 55 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 500.0 ml - -
Solution B 300.0 ml - -
Solution C 200.0 ml - -
Agar , if required 1.5 % 15 44.599
1 Autoclave solutions A and B separately, filter sterilize solution C. Add 1.5% agar if necessary.

Solution A #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 12 H2O 9.0 g 18 126.797
KH2PO4 1.5 g 3 22.045
NH4Cl 1.0 g 2 37.39
MgSO4 x 7 H2O 0.2 g 0.4 1.623
Trace element solution SL-6 0.3 ml - -
Distilled water 500.0 ml - -

Solution B #

300
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 0.01 g 0.033 0.227
Ferric ammonium citrate 5.00 mg 0.017 0.064
Distilled water 300.00 ml - -

Solution C #

200
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fructose 5 g 25 138.766
Distilled water 200 ml - -

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 15.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 3
Cupriavidus necator DSM 422

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Na2HPO4 x 12 H2O 9 63.399
Fructose 5 27.753
KH2PO4 1.5 11.023
NH4Cl 1 18.695
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
temperature mesophilic 3 strains