DSMZ 585 . BCYE AGAR

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
OXOID Legionella CYE-Agar base
(with Legionella BCYE supplement)
1000 ml - -
1 Dehydrated media preparations are available from media distributors.
2 We use OXOID Legionella CYE-Agar base with Legionella BCYE supplement.
3 For solubilization of the lyophilized pellet use standard liquid media. Although Legionellae do not grow in those media they are suitable for resuspension and transfer onto agar plates.
4 Note: Alternatively use CYE-ACES AGAR
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 490 ml - -
Solution B 490 ml - -
Solution C 10 ml - -
Solution D 10 ml - -
1 Add Cysteine-HCl, then ferric pyrophosphate solution to solution A. Adjust the complete medium to pH 6.9 ± 0.5 at 50°C with sterile 1.0 N KOH or HCl. The pH of the medium is critical. Finally, add solution B. Swirl medium in flask during dispensing to petri dishes or tubes in order to keep charcoal suspended.
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract
(Difco 0127)
10 g 20.408 -
ACES
(N-2-acetamido-2-aminoethane-sulfonic acid)
10 g 20.408 112.01
Activated charcoal
(Sigma C 5510)
2 g 4.082 -
Distilled water 490 ml - -
1 Dissolve ACES and bring to pH 6.9 by slowly adding 1 N KOH. Add the other components and dissolve by boiling. Autoclave at 121°C for 15 minutes. Cool to 50 - 55°C.
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Agar 15 g 30.612 91.019
Distilled water 490 ml - -
1 Autoclave at 121°C and cool to 50 - 55°C.
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Cysteine HCl x H2O 0.4 g 40 227.744
Distilled water 10.0 ml - -
1 Prepare a fresh solution and filter-sterilize it.
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fe4(PO4)2 0.25 g 25 33.548
Distilled water 10.00 ml - -
1 Prepare a fresh solution by heating to 50 - 55°C in a water-bath. Filter-sterilize solution separately.
2 Please note: Soluble ferric pyrophosphate must be kept dry and in the dark. Do not use if chemical looses its green colour and becomes brown or yellow.

Metadata

Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 108
Strains with modifications: 90

Molecular composition

Compound Conc. [g/L] Conc. [mM]
OXOID Legionella CYE-Agar base - -

Cultivation metadata from 108 strains BacDive

pH 4 - 6 2 strains
pH 6 - 8 1 strain
phyla 1 112 strains
oxygen microaerophile 86 strains
oxygen aerobe 8 strains
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