MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 619 . HYPHOMICROBIUM METHYLOVORUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Medium for Hyphomicrobium methylovorum DSM 5458

This strain uses the standard medium without modifications.

Growth conditions: 30 °C

Main sol. 619 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Methanol 10.0 ml 7.92 247.176
(NH4)2HPO4 3.0 g 3 22.717
NaCl 1.0 g 1 17.112
MgSO4 x 7 H2O 0.2 g 0.2 0.811
FeSO4 x 7 H2O 10.0 mg 0.01 0.036
MnSO4 x 1 H2O 5.0 mg 0.005 0.033
Vitamin mixture 5.0 ml - -
Agar 15.0 g 15 44.599
Tap water 1000.0 ml - -
1 Sterilize methanol by filtration, and add to other components sterilized by autoclaving.

Vitamin mixture #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 0.2 mg 2.0e-4 8.2e-4
Calcium pantothenate 40.0 mg 0.04 0.084
Folic acid 0.2 mg 2.0e-4 4.5e-4
Inositol 200.0 mg 0.2 1.11
Niacin 40.0 mg 0.04 0.325
p-Aminobenzoic acid 20.0 mg 0.02 0.146
Pyridoxine hydrochloride 40.0 mg 0.04 0.195
Riboflavin 40.0 mg 0.04 0.106
Thiamine-HCl x 2 H2O 20.0 mg 0.02 0.054
Choline 100.0 mg 0.1 0.96
Vitamin B12 2.0 µg 2.0e-6 1.5e-6
Distilled water 1000.0 ml - -

Metadata

Last modified: 15.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Filter
Edit in Medium builder

Associated strains

All strains for this medium: 1
Hyphomicrobium methylovorum DSM 5458

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Methanol 7.92 247.176
(NH4)2HPO4 3 22.717
NaCl 1 17.112
MgSO4 x 7 H2O 0.2 0.811
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain