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DSMZ

DSMZ 684 . SYNTROPHOBACTER (MPOB) MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Trichococcus flocculiformis DSM 23957

* Modification: Replace Na2-fumarate with glycerol 2.3 g/L; anaerobic

Main sol. 684 #

ml
Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 0.53 g 0.528 3.722
KH2PO4 0.41 g 0.409 3.004
NH4Cl 0.30 g 0.299 5.592
CaCl2 x 2 H2O 0.11 g 0.11 0.746
MgCl2 x 6 H2O 0.10 g 0.1 0.49
NaCl 0.30 g 0.299 5.118
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Yeast extract 0.20 g 0.199 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
Na2CO3 1.50 g 1.496 14.11
Na2-fumarate* 3.20 g 3.19 19.936
Wolin's vitamin solution (10x) 1.00 ml - -
Na2S x 9 H2O 0.50 g 0.499 2.076
Glycerol* 2.30 g - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except carbonate, vitamins, fumarate and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving complete the medium by adding vitamins, fumarate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Stock solutions of vitamins and fumarate are sterilized by filtration. Adjust pH of the complete medium to 7.0 - 7.2. After inoculation pressurize culture vials to 0.7 bar overpressure with sterile 80% N2 and 20% CO2 gas mixture.
2 Note: A white precipitate forms in this medium after autoclaving, which has however no negative effect on growth.

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Selenite-tungstate solution #

1000
Solution occurs in 178 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 06.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.0 - 7.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 7
Strains with modifications: 6
Trichococcus flocculiformis DSM 23957

  Na2-fumarate
  2.3 g Glycerol

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Glycerol 2.3 24.976
Na2CO3 1.496 14.11
Na2HPO4 x 2 H2O 0.528 3.722
Na2S x 9 H2O 0.499 2.076
KH2PO4 0.409 3.004
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Cultivation metadata from 7 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 4 8 strains
oxygen anaerobe 7 strains
oxygen obligate anaerobe 1 strain
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