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784. NATRONIELLA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.2 g 0.197 1.447
2 0.1 g 0.098 0.484
3 1.0 g 0.984 18.4
4 0.2 g 0.197 2.64
5 16.0 g 15.748 269.462
6 Trace element solution SL-11 1.0 ml - -
7 0.2 g 0.197 -
8
(0.1% w/v)
0.5 ml 4.9e-4 0.002
9 68.0 g 66.929 631.478
10 38.0 g 37.402 445.222
11 5.0 ml 3.888 84.392
12 Vitamin solution 10.0 ml - -
13 1.0 g 0.984 4.098
14 1000.0 ml 982.487 54536.3
15 , might need strain adjustment 2.0 g/l - -
Instructions:
  1. Dissolve ingredients except carbonate, bicarbonate, ethanol, vitamins and sulfide. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonates and sulfide while gassing the head space only. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use add ethanol and vitamins from sterile anoxic stock solution prepared under 100% N2 gas. Vitamins are sterilized by filtration. Adjust pH of the complete medium to 9.5 - 10.0, if necessary.
Solution occurs in 22 other media (first defined in medium 722 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.2 g 5.2 13.97
2 1.5 g 1.5 7.545
3 70.0 mg 0.07 0.514
4 100.0 mg 0.1 0.505
5 6.0 mg 0.006 0.097
6 190.0 mg 0.19 0.799
7 2.0 mg 0.002 0.012
8 24.0 mg 0.024 0.101
9 36.0 mg 0.036 0.149
10 1000.0 ml 998.207 55408.8
Instructions:
  1. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 10.0
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 2
Strains with modifications: 2

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 993.294 55136.2
Na2CO3 66.929 631.478
NaHCO3 37.402 445.222
NaCl 15.748 269.462
Ethanol 3.888 84.392
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Cultivation metadata from 2 strains BacDive

halophily moderate 1 strain
halophily not 1 strain
pH 10 - 14 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
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784a. METHANOCALCULUS NATRONOPHILUS MEDIUM 92 %
1352. FUCHSIELLA ALKALIACETIGENA MEDIUM 90 %
1297. NATRONIELLA SULFIDIGENA MEDIUM 88 %
932. HALONATRONUM SACCHAROPHILUM MEDIUM 88 %
1505. PROTEINIVORAX MEDIUM 87 %

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