DSMZ 784 . NATRONIELLA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Gassing station Autoclave Hungate tubes Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.2 g 0.197 1.447
MgCl2 x 6 H2O 0.1 g 0.098 0.484
NH4Cl 1.0 g 0.984 18.401
KCl 0.2 g 0.197 2.641
NaCl 16.0 g 15.748 269.474
Trace element solution SL-11 1.0 ml - -
Yeast extract 0.2 g 0.197 -
Sodium resazurin
(0.1% w/v)
0.5 ml 4.9e-4 0.002
Na2CO3 68.0 g 66.929 631.478
NaHCO3 38.0 g 37.402 445.22
Ethanol absolute 5.0 ml - -
Wolin's vitamin solution 10.0 ml - -
Na2S x 9 H2O 1.0 g 0.984 4.098
Distilled water 1000.0 ml - -
1 Dissolve ingredients except carbonate, bicarbonate, ethanol, vitamins and sulfide. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonates and sulfide while gassing the head space only. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use add ethanol and vitamins from sterile anoxic stock solution prepared under 100% N2 gas. Vitamins are sterilized by filtration. Adjust pH of the complete medium to 9.5 - 10.0, if necessary.
Solution occurs in 22 other media (first defined in Medium 722).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA x 2 H2O 5.2 g 5.2 15.467
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 1000.0 ml - -
1 Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 19.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.5 - 10.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 66.929 631.478
NaHCO3 37.402 445.22
NaCl 15.748 269.474
NH4Cl 0.984 18.401
Na2S x 9 H2O 0.984 4.098
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Cultivation metadata from 2 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
pH 10 - 12 1 strain
phyla 1 2 strains
oxygen anaerobe 2 strains
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