791
791 .
PORPHYROBACTER TEPIDARIUS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Tepidimonas sp. DSM 14591
This strain uses the standard medium without modifications.
Growth conditions: 50 °C
Main sol. 791 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na glutamate | 0.5 | g/l | 0.5 | 2.957 | |
| Sodium succinate | 0.5 | g/l | 0.5 | 3.085 | |
| Sodium acetate | 0.5 | g/l | 0.5 | 6.095 | |
| Yeast extract | 0.5 | g/l | 0.5 | - | |
| Casamino acids | 0.5 | g/l | 0.5 | - | |
| (NH4)2SO4 | 0.5 | g/l | 0.5 | 3.784 | |
| Sodium thiosulphate | 0.5 | g/l | 0.5 | 3.162 | |
| Basal salts solution | 5.0 | ml | - | - | |
| Phosphate solution | 5.0 | ml | - | - | |
| Vitamin solution | 1.0 | ml | - | - | |
| Distilled water | 989.0 | ml | - | - | |
| 1 Final pH 7.5 | |||||
| 2 Add the vitamin solution, aseptically to the cooled, autoclaved medium. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Na3-EDTA | 4.53 | g/l | 4.53 | 12.647 |
| FeSO4 x 7 H2O | 1.11 | g/l | 1.11 | 3.993 |
| MgSO4 x 7 H2O | 24.65 | g/l | 24.65 | 100.01 |
| CaCl2 x 2 H2O | 2.94 | g/l | 2.94 | 19.998 |
| NaCl | 23.40 | g/l | 23.4 | 400.411 |
| MnSO4 x 4 H2O | 112.00 | mg/l | 0.112 | 0.502 |
| ZnSO4 x 7 H2O | 29.00 | mg/l | 0.029 | 0.101 |
| Co(NO3)2 x 6 H2O | 29.00 | mg/l | 0.029 | 0.1 |
| CuSO4 x 5 H2O | 25.00 | mg/l | 0.025 | 0.1 |
| Na2Mo4 x 2 H2O | 24.00 | mg/l | 0.024 | - |
| H3BO3 | 31.00 | mg/l | 0.031 | 0.501 |
| Distilled water | 1000.00 | ml | - | - |
Solution occurs in 4
other media (first defined in Medium J303).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| KH2PO4 | 75 | g | 75 | 551.126 |
| K2HPO4 | 78 | g | 78 | 447.823 |
| Distilled water | 1000 | ml | - | - |
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nicotinic acid | 100 | mg | 1 | 8.123 | |
| Thiamine | 100 | mg | 1 | 3.768 | |
| Biotin | 5 | mg | 0.05 | 0.205 | |
| p-Aminobenzoic acid | 50 | mg | 0.5 | 3.646 | |
| Vitamin B12 | 1 | mg | 0.01 | 0.007 | |
| Calcium pantothenate | 50 | mg | 0.5 | 1.049 | |
| Pyridoxine hydrochloride | 50 | mg | 0.5 | 2.431 | |
| Folic acid | 50 | mg | 0.5 | 1.133 | |
| Na3-EDTA | 200 | mg | 2 | 5.584 | |
| Distilled water | 100 | ml | - | - | |
| 1 Filter sterilize. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.5 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 5 |
Tepidimonas sp. DSM 14591
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sodium acetate | 0.5 | 6.095 |
| (NH4)2SO4 | 0.5 | 3.784 |
| Sodium thiosulphate | 0.5 | 3.162 |
| Sodium succinate | 0.5 | 3.085 |
| Na glutamate | 0.5 | 2.957 |
| Show more | ||
