806
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THERMOCOCCUS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Thermococcus guaymasensis DSM 11113
This strain uses the standard medium without modifications.
Growth conditions: 88 °C
Main sol. 806 #
ml
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KCl | 0.33 | g | 0.33 | 4.427 | |
| MgCl2 x 2 H2O | 2.70 | g | 2.7 | 20.573 | |
| MgSO4 x 7 H2O | 3.40 | g | 3.4 | 13.794 | |
| NH4Cl | 0.25 | g | 0.25 | 4.674 | |
| CaCl2 x 2 H2O | 0.14 | g | 0.14 | 0.952 | |
| K2HPO4 | 0.14 | g | 0.14 | 0.804 | |
| Na2SeO3 | 1.00 | µg | 1.0e-6 | 5.8e-6 | |
| NiCl2 x 6 H2O | 1.00 | µg | 1.0e-6 | 7.7e-6 | |
| NaHCO3 | 1.00 | g | 1 | 11.904 | |
| NaCl | 18.00 | g | 18 | 308.008 | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
| Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
| Yeast extract | 1.00 | g | 1 | - | |
| Trypticase | 1.00 | g | 1 | - | |
| Sulphur | 5.00 | g | 5 | - | |
| Modified Wolin's mineral solution II | 10.00 | ml | - | - | |
| Wolin's vitamin solution | 10.00 | ml | - | - | |
| Distilled water | 980.00 | ml | - | - | |
| 1 Prepare the medium without NaHCO3, cysteine hydrochloride, Na2S x 9 H2O, and vitamin solution. Boil the medium and cool under N2. Add the NaHCO3 to the cooled medium and adjust the pH to about pH 7.2. Sterilise the medium at 100°C for 3 hours on 3 consecutive days. Reduce the medium by adding 10% neutralised sodium sulphide and cysteine hydrochloride from sterile stock solutions. The vitamins are added from a sterile (filter sterilised) stock solution prepared under nitrogen. The final pH is 7.2. | |||||
Solution occurs in 24
other media (first defined in Medium 700).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.500 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.000 | g | 3 | 12.172 | |
| MnSO4 x H2O | 0.500 | g | 0.5 | 2.958 | |
| NaCl | 1.000 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.100 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.180 | g | 0.18 | 1.161 | |
| CaCl2 x 2 H2O | 0.100 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.180 | g | 0.18 | 0.626 | |
| CuSO4 x 5 H2O | 0.010 | g | 0.01 | 0.04 | |
| AlK(SO4)2 x 12 H2O | 0.020 | g | 0.02 | 0.042 | |
| H3BO3 | 0.010 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.010 | g | 0.01 | 0.041 | |
| NiCl2 x 6 H2O | 0.025 | g | 0.025 | 0.193 | |
| Na2SeO3 x 5 H2O | 0.300 | mg | 3.0e-4 | 0.001 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. | |||||
Solution occurs in 53
other media (first defined in Medium 141).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium D-(+)-pantothenate | 5.0 | mg | 0.005 | 0.021 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| (DL)-alpha-Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Thermococcus guaymasensis DSM 11113
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sulphur | 5 | - |
| MgCl2 x 2 H2O | 2.7 | 20.573 |
| NaHCO3 | 1 | 11.904 |
| Trypticase | 1 | - |
| Yeast extract | 1 | - |
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