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Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
MgSO4 x 7 H2O | 17.00 | g | 17 | 68.972 | |
NaCl | 100.00 | g | 100 | 1711.16 | |
Solution A | 50.00 | ml | - | - | |
Solution B | 50.00 | ml | - | - | |
Trace element solution SL-10 | 10.00 | ml | - | - | |
Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
Yeast extract | 10.00 | g | 10 | - | |
Trypticase | 10.00 | g | 10 | - | |
Glucose | 5.00 | g | 5 | 27.753 | |
Na2S x 9 H2O | 0.25 | g | 0.25 | 1.041 | |
NaHCO3 | 4.10 | g | 4.1 | 48.806 | |
L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
Distilled water | 890.00 | ml | - | - | |
1 pH 7.0 | |||||
2 Prepare the medium without yeast extract, glucose, NaHCO3, cysteine hydrochloride and Na2S x 9 H2O. Boil and then cool under a gas atmosphere of N2:CO2 (80:20 v/v) and add the NaHCO3 and cysteine hydrochloride. Autoclave the medium and add from sterile stock solutions yeast extract, trypticase, glucose, and Na2S x 9 H2O. The final pH should be 7.0. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
KH2PO4 | 6.00 | g | - | - |
(NH4)2SO4 | 6.00 | g | - | - |
NaCl | 12.00 | g | - | - |
MgSO4 x 7 H2O | 2.60 | g | - | - |
NH4Cl | 2.50 | g | - | - |
CaCl2 x 2 H2O | 0.28 | g | - | - |
K2HPO4 | 0.28 | g | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
Distilled water | 990.0 | ml | - | - | |
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.0 |
Equipment needed: | Autoclave |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 100 | 1711.16 |
MgSO4 x 7 H2O | 17 | 68.972 |
Trypticase | 10 | - |
Yeast extract | 10 | - |
Glucose | 5 | 27.753 |
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