838
838 .
GEOANAEROBACTER MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 838 #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Ferric citrate monohydrate | 10.0 | g | 9.98 | 37.953 | |
| KH2PO4 | 0.6 | g | 0.599 | 4.4 | |
| NH4Cl | 0.3 | g | 0.299 | 5.597 | |
| MgSO4 x 7 H2O | 0.5 | g | 0.499 | 2.025 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.679 | |
| Trace element solution SL-11 | 1.0 | ml | - | - | |
| Selenite-tungstate solution | 1.0 | ml | - | - | |
| Na2CO3 | 1.5 | g | 1.497 | 14.124 | |
| Na-acetate | 0.8 | g | 0.798 | 9.733 | |
| Na-ascorbate | 0.8 | g | 0.798 | 4.03 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 First dissolve ferric citrate by heating the water under continuous stirring. After cooling to room temperature adjust the pH to 6.0, then add and dissolve the remaining ingredients, except carbonate, acetate and ascorbate. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add acetate and ascorbate from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to inoculation check the medium pH and adjust to 6.8, if necessary. | |||||
Solution occurs in 22
other media (first defined in Medium 722).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2-EDTA x 2 H2O | 5.2 | g | 5.2 | 15.467 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml. | |||||
Solution occurs in 179
other media (first defined in Medium 385).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaOH | 0.5 | g | 0.5 | 12.501 |
| Na2SeO3 x 5 H2O | 3.0 | mg | 0.003 | 0.011 |
| Na2WO4 x 2 H2O | 4.0 | mg | 0.004 | 0.012 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 23.05.23 |
| Source: | DSMZ |
| Synonyms: | GEOBACTER PELOPHILUS MEDIUM |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 6.8 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Ferric citrate monohydrate | 9.98 | 37.953 |
| Na2CO3 | 1.497 | 14.124 |
| Na-acetate | 0.798 | 9.733 |
| Na-ascorbate | 0.798 | 4.03 |
| KH2PO4 | 0.599 | 4.4 |
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