DSMZ 926 . ALKALIPHILIC THERMOCOCCUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Equipment needed: Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 27.70 g 13.85 236.995
MgSO4 x 7 H2O 7.00 g 3.5 14.2
MgCl2 x 6 H2O 5.50 g 2.75 13.527
KCl 0.65 g 0.325 4.359
NaBr 0.10 g 0.05 0.486
NaHCO3 0.32 g 0.16 1.905
K2HPO4 1.00 g 0.5 2.871
CaCl2 x 2 H2O 0.05 mg 2.5e-5 1.7e-4
KI 15.00 mg 0.008 0.029
H3BO3 0.03 g 0.015 0.243
Modified Wolin's mineral solution II 20.00 ml - -
Distilled water 2000.00 ml - -
1 Prepare the medium anaerobically under nitrogen. Do not adjust the pH.
2 Prepare separate anaerobic stock solutions of, Casamino acids(10%), Yeast extract (10%), and glycine (2M = 150 g/l). A 0.5M polysulphide solution is prepared by dissolving 12.0 g Na2S x 9 H2O in oxygen free water, followed by adding 1.6 g sulphur - the solution will be dark yellow.
Solution occurs in 22 other media (first defined in Medium 700).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g - -
MgSO4 x 7 H2O 3.000 g - -
MnSO4 x H2O 0.500 g - -
NaCl 1.000 g - -
FeSO4 x 7 H2O 0.100 g - -
CoSO4 x 7 H2O 0.180 g - -
CaCl2 x 2 H2O 0.100 g - -
ZnSO4 x 7 H2O 0.180 g - -
CuSO4 x 5 H2O 0.010 g - -
AlK(SO4)2 x 12 H2O 0.020 g - -
H3BO3 0.010 g - -
Na2MoO4 x 2 H2O 0.010 g - -
NiCl2 x 6 H2O 0.025 g - -
Na2SeO3 x 5 H2O 0.300 mg - -
Distilled water 1000.000 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 15.02.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Anaerobic workbench
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Associated strains

All strains for this medium: 2
Strains with modifications: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 2.75 13.527
K2HPO4 0.5 2.871
KCl 0.325 4.359
NaHCO3 0.16 1.905
NaBr 0.05 0.486
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
temperature hyperthermophilic 2 strains