C99
99 .
S/W
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Medium for Paramecium primaurelia CCAP1660/2A
* Modification: plus wheat grain
Mail sol. C99 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Soil
(air-dried, sieved, garden loam) |
trace | g | - | - | |
| 1 Put a layer about 1 cm deep of air-dried, sieved good calcareous garden loam into a test tube or jar. (The use of mud from rivers or ponds is rarely satisfactory.) Carefully add deionised water to a depth of 7 to 10 cm, plug or cover, and steam for one hour or autoclave for 15 minutes at 15 psi (longer for larger vessels) on each of 2 consecutive days; further sterilisation is not needed. Allow to stand for a further day before inoculating, when the pH should be between 7.0 - 8.0. | |||||
Metadata
| Last modified: | 07.10.22 |
| Source: | CCAP |
| Synonyms: | Soil/Water Biphasic Medium |
| Medium type: | Complex medium |
| Final pH: | 7.0 - 8.0 |
| Reference: | doi.org/10.2307/2256465 |
| Edit in Medium builder | |