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JCM 336 . PE MEDIUM (pH 6.8)

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J336 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Medium J303 1000 ml - -
1 Adjust pH to 6.8.

Medium J303 #

1011
Solution occurs in 2 other media (first defined in Medium J303).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Basal salts solution 5.0 ml - -
Phosphate solution 5.0 ml - -
Vitamin solution 1.0 ml - -
(NH4)2SO4 0.5 g 0.495 3.743
Na glutamate 0.5 g 0.495 2.588
Sodium succinate 0.5 g 0.495 3.052
Sodium acetate 0.5 g 0.495 6.029
Yeast extract
(BD-Difco) 		0.5 g 0.495 -
Casamino acids
(BD-Difco) 		0.5 g 0.495 -
Na2S2O3 0.5 g 0.495 3.128
Distilled water 1000.0 ml - -
1 Adjust pH to 7.5.

Basal salts solution #

1010
Solution occurs in 3 other media (first defined in Medium J303).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na3-EDTA 4.12 g 4.079 -
FeSO4 x H2O 1.11 g 1.099 6.468
MgSO4 x 7 H2O 24.65 g 24.406 99.02
CaCl2 x 2 H2O 2.94 g 2.911 19.8
NaCl 23.40 g 23.168 396.446
Trace elements solution 10.00 ml - -
Distilled water 1000.00 ml - -

Phosphate solution #

1000
Solution occurs in 4 other media (first defined in Medium J303).
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 75 g 75 551.126
K2HPO4 78 g 78 447.823
Distilled water 1000 ml - -

Vitamin solution #

100
Solution occurs in 3 other media (first defined in Medium J303).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nicotinic acid 100 mg 1 8.123
Thiamine HCl 100 mg 1 -
Biotin 5 mg 0.05 0.205
p-Aminobenzoic acid 50 mg 0.5 3.646
Vitamin B12 1 mg 0.01 0.007
Calcium pantothenate 50 mg 0.5 1.049
Pyridoxine hydrochloride 50 mg 0.5 -
Folic acid 50 mg 0.5 1.133
Na3-EDTA 200 mg 2 -
Distilled water 100 ml - -

Metadata

Last modified: 03.04.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
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Associated strains

All strains for this medium: 1

Media similar to medium J336

J172 SULFOLOBUS MEDIUM (B)
J219 CYC AGAR (pH 8.0)
J337 PE MEDIUM (pH 7.2)
J350 THERMOCOCCUS MEDIUM (pH 6.0)
J1132 HALOBACTERIA MEDIUM (pH 8.0)
585 BCYE AGAR
672 HALF STRENGTH NUTRIENT BROTH OR AGAR
C71 NSW
C99 S/W
C58 MW
C100 S/W + AMP

Molecular composition

Compound Conc. [g/L] Conc. [mM]
- -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature thermophilic 1 strain