J1033
1033 .
ST65 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1033 #
ml
Equipment needed: Anaerobic workbench
Hungate tubes
Autoclave
| 1 Mix components, except sulfur, bring to a boil and cool down under a CO2 (100%) gas stream. While gassing, add the following components to the medium: | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 18.000 | g | 17.476 | 299.037 | |
| MgCl2 x 6 H2O | 4.330 | g | 4.204 | 20.678 | |
| NH4Cl | 0.330 | g | 0.32 | 5.99 | |
| KCl | 0.330 | g | 0.32 | 4.298 | |
| KH2PO4 | 0.165 | g | 0.16 | 1.177 | |
| CaCl2 x 2 H2O | 0.033 | g | 0.032 | 0.218 | |
| Trace elements solution | 10.000 | ml | - | - | |
|
Sulfur
(powder) |
5.000 | g | 4.854 | 151.368 | |
| Distilled water | 1000.000 | ml | - | - | |
| Trace vitamins | 10.000 | ml | - | - | |
| NaHCO3 | 2.000 | g | 1.942 | 23.114 | |
| 2 Bubble CO2 for 3 - 4 min and check pH to be 6.5 - 6.8. Readjust pH to the range if necessary. Autoclave under a CO2 gas atmosphere. Add the following solution to the medium (separately autoclaved under N2): | |||||
|
KNO3
(1 M) |
10.000 | ml | - | - | |
| 3 Steam sulfur for 3 hr on each of 3 successive days. Aseptically and anaerobically distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 10 ml of the medium and 0.05 g sulfur in Hungate tubes) under a CO2 stream and seal with butyl rubber stoppers. | |||||
Solution occurs in 3
other media (first defined in Medium J1030).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Fe(NH4)2(SO4)2 x 6 H2O | 78.40 | mg | 0.078 | - |
| CoCl2 x 6 H2O | 23.80 | mg | 0.024 | 0.183 |
| NiCl2 x 6 H2O | 16.60 | mg | 0.017 | 0.128 |
| Na2MoO4 x 2 H2O | 2.42 | mg | 0.002 | 0.01 |
| Na2WO4 x 2 H2O | 5.49 | mg | 0.005 | 0.017 |
| ZnSO4 x 7 H2O | 14.40 | mg | 0.014 | 0.05 |
| CuCl2 x 2 H2O | 0.17 | mg | 1.7e-4 | 1.0e-3 |
| Na2SeO4 | 9.45 | mg | 0.009 | 0.05 |
| H3BO3 | 0.62 | mg | 6.2e-4 | 0.01 |
| MnCl2 x 4 H2O | 19.80 | mg | 0.02 | - |
| SrSO4 | 0.92 | mg | 9.2e-4 | - |
| CrK(SO4)2 x 12 H2O | 0.50 | mg | 5.0e-4 | - |
| AlCl3 x 6 H2O | 2.41 | mg | 0.002 | 0.01 |
| BaCl2 | 2.08 | mg | 0.002 | 0.01 |
| Na2SiO3 x 9 H2O | 14.20 | mg | 0.014 | - |
| KBr | 11.90 | mg | 0.012 | 0.1 |
| KI | 16.60 | mg | 0.017 | 0.064 |
| Na2SO4 | 71.00 | mg | 0.071 | 0.5 |
| Distilled water | 1000.00 | ml | - | - |
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Medium type: | Defined medium |
| Final pH: | n.d. |
| Equipment needed: | Anaerobic workbench, Autoclave, Hungate tubes |
| Edit in Medium builder | |